| 番茄基因组DNA不同微量提取方法的比较及适用性分析 |
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| 引用本文：王海英，刘航空.番茄基因组DNA不同微量提取方法的比较及适用性分析[J].西北农业学报,2022,(12):1560~1567 |
| DOI:10.7606/j.issn.1004-1389.2022.12.004 |
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| 基金项目:陕西省科技计划重点研发项目（2021NY-049）。 |
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| 中文摘要:为了探究不同基因组DNA提取方法在生物学试验中适用性问题，采用5种方法提取番茄基因组DNA，并通过琼脂糖凝胶电泳、微量紫外分光光度计、聚合酶链式反应（PCR）比较提取的DNA浓度、质量及适用性。结果显示，碱裂解煮沸法成本低、操作简便，但所提取的DNA浓度最低，质量最差，只适合一般PCR检测试验；SDS冰浴法和改良的CTAB法操作步骤多、程序复杂，提取的DNA浓度和质量好，通用性最强，但所用试剂具有毒性；高盐低pH法和PVP-40法操作简单，提取的DNA浓度和质量较好，具有一定的通用性，且所用试剂无毒，建议一般性的生物学试验可优先考虑这两种方法。 |
| 中文关键词:番茄 DNA提取方法 适用性分析 |
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| Comparison of Different DNA Extraction Methods and Analysis of Its Applicability in Tomato |
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| Abstract:In order to explore the applicability of different DNA extraction methods in biological experiments, we used five common methods to extract chromosome DNA in tomato(Solanum lycopersicum L.), and then we compared the DNA concentration, quality and applicability among these methods by using agarose gel electrophoresis, microultraviolet spectrophotometer and polymerase chain reaction (PCR). The results showed that the alkaline lysis method was low in cost and easy to operate, however, the DNA concentration was the lowest and the quality was the worst. It was not universal and only suitable for general molecular detection by PCR. The SDS method and the improved CTAB method had multiple operation steps and complicated procedures. The concentration and quality of DNA were the best for almost all biological experiments, but the reagent used was toxic. The high salt with low pH method and PVP-40 method had simple operation steps, and the DNA with good concentration and quality had certain commonability. Moreover, the reagents used in this experiment were non-toxic. It is suggested that these two methods can be given priority in general biological experiments. |
| keywords:Tomato DNA extraction methods Applicability |
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