| 花魔芋响应软腐病菌侵染初期的转录组分析 |
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| 引用本文：何 斐，冯明月，王菁轩，邵永春，王 甜，王 莹，卢谢敏，方姝颖.花魔芋响应软腐病菌侵染初期的转录组分析[J].西北农业学报,2022,30(6):777~786 |
| DOI:10.7606/j.issn.1004-1389.2022.06.009 |
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| 基金项目:国家自然科学基金（31901468）；陕西省创新人才推进计划（2020KJXX-003）；陕西省重点研发计划（2021NY-048）；农业部富硒产品开发与质量控制重点实验室（Se-2019B02）；中国富硒产业研究院富硒专项研发计划（2019QCY-2.2）；安康市科技计划(AK2020-CQ01-1)；安康市农业技术推广项目（2017AYHX016）。 |
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| 中文摘要:为探讨花魔芋抵御软腐病害的分子机制，分别以染病初期和健康的花魔芋球茎为材料进行转录组测序。结果表明，健康和染病初期花魔芋两组出现3 222个差异表达基因，其中2 660个基因上调表达，562个基因下调表达，差异表达基因主要涉及细胞组分、生物学过程及分子功能等生理生化过程。表达量上调和下调最大的前10个基因包含编码MiAMP1抗菌蛋白、热激蛋白、胰蛋白酶与蛋白酶抑制剂、胰凝乳蛋白酶抑制剂等与抗病相关的基因。GO功能分类和KEGG通路分析表明，类黄酮和苯丙烷类物质在花魔芋抵御软腐病菌侵染初期发挥重要作用。值得关注的是，硒化合物代谢、维生素B6代谢、类黄酮生物合成、N-聚糖生物合成及链霉素生物合成通路等抗病相关的差异表达基因在花魔芋染病初期全部或大部分受诱导表达。利用高通量测序获得大量花魔芋转录组信息，有助于挖掘与花魔芋抗软腐病相关的关键基因，也为魔芋抗病分子育种提供理论参考。 |
| 中文关键词:花魔芋 软腐病 转录组 差异表达基因 |
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| Transcriptomic Analysis of Amorphophallus konjac With Early Response to Soft Rot Pathogen Infection |
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| Abstract:Soft rot caused by Pectobacterium chrysanthemi has become the most serious bacterial disease that threatens Amorphophallus konjac production,and it is difficult to control soft rot under field conditions. In order to explore the molecular mechanisms which underpin the response of A.konjac to soft rot disease, transcriptome analysis of healthy and infected corms was performed in this study. The results indicated that 3 222 genes were differentially expressed in A.konjac at the early stage of infection with P.chrysanthemi. Among them,2 660 genes were up-regulated,whereas 562 genes were down-regulated upon infection. The top 10 up and down-regulated genes included genes encoding the plant antimicrobial protein MiAMP1,heat shock protein,trypsin and protease inhibitor,chymotrypsin inhibitor,and genes associated with plant disease resistance. GO annotation and KEGG pathway enrichment analysis of the differentially expressed genes revealed that flavonoid and phenylpropanoid might be involved in the defense response of A.konjac to early infection. All or most of the differentially expressed genes involved in resistance-related pathways (e.g.,selenocompound metabolism,vitamin B6 metabolism,flavonoid biosynthesis,N-glycan biosynthesis,and streptomycin biosynthesis) were induced during early infection. A large amount of transcriptome information from A.konjac was obtained in this study by high-throughput sequencing,which could be useful for the identification of key genes related to plant defense and genetic breeding of plant resistance to the soft rot disease. |
| keywords:Amorphophallus konjac Soft rot disease Transcriptome Differentially expressed genes |
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