| IGF-1、bFGF、ITS、2-ME2对牛耳皮肤成纤维细胞细胞周期的影响 |
| 点此下载全文 |
| 引用本文：孟晓俣，刘 欣，孙 羽，张广辉，张 涌.IGF-1、bFGF、ITS、2-ME2对牛耳皮肤成纤维细胞细胞周期的影响[J].西北农业学报,2022,(5):552~558 |
| DOI:10.7606/j.issn.1004-1389.2022.05.003 |
| 摘要点击次数: 83 |
| 全文下载次数: 26 |
|
| 基金项目:陕西省胚胎工程中心项目（20190926000003）。 |
|
| 中文摘要:为探讨2-甲氧雌二醇（2-Methoxyestradiol，2-ME2）、胰岛素样生长因子-1（Insulin-like Growth Factor 1，IGF-1）、碱性成纤维生长因子 （basic Fibroblast Growth Factor，bFGF）、胰岛素-转铁蛋白-硒（Insulin-Transferrin-Selenium，ITS）对体细胞细胞周期的影响，以牛（Bos taurus）耳皮肤成纤维细胞为研究对象，进行流式细胞分析和细胞计数。结果显示，1 μmol/L 2-ME2可显著提高细胞周期中G0/G1期细胞及G1早期（early G1，eG1）细胞所占比例，20 ng/mL IGF-1、10 ng/mL bFGF、ITS及4种细胞因子联合处理对细胞周期中G0/G1期细胞和G1早期细胞所占比例无显著影响，表明2-ME2能够显著影响牛耳皮肤成纤维细胞的细胞周期，研究结果对体细胞核移植技术在短时间内获得更多G0/G1期和eG1期的供体细胞具有重要意义。 |
| 中文关键词:G1早期 细胞周期 体细胞核移植 牛耳皮肤成纤维细胞 |
| |
| Effects of IGF-1, bFGF, ITS, 2-ME2 on the Cell Cycle of Bovine Ear Skin Fibroblasts |
|
|
| Abstract:In order to explore the effects of Insulin-like Growth Factor 1(IGF-1), basic Fibroblast Growth Factor (bFGF), Insulin-Transferrin-Selenium (ITS) and 2-Methoxyestradiol (2-ME2) on somatic cells cycle, this experiment using flow cytometry and cell counting took Bos taurus ear fibroblasts as the research object. The results showed that 1 μmol/L 2-ME2 significantly increased the proportion of cells in the G0/G1 and early G1 (eG1) phase of the cell cycle. 20 ng/mL IGF-1, 10 ng/mL bFGF and ITS have no effect on the proportion of G0/G1 cells and early G1 cells in the cell cycle. The study showed that 2-ME2 can significantly affect the cell cycle of bovine ear fibroblasts. This study is of great significance for obtaining more donor cells for somatic cell nuclear transfer in G0/G1 phase and eG1 phase in a short period of time. |
| keywords:Early G1 phase Cell cycle Somatic cell nuclear transfer Bovine ear fibroblast cells |
| 查看全文 查看/发表评论 下载PDF阅读器 |
