| Abstract:In order to investigate the preventive effect of traditional Chinese medicine extractive (TCME) on SPF chicks inoculated with IBDV. 150 SPF chicks were randomly divided into control group,model group,TCME high,medium and low dose groups. In groups of TCME high,medium and low dose,extractive doses of 10.0,5.0,2.5 g/L were respectively added into drinking water for 7 consecutive day, but no drug was added in the control group and model group.Except for control group,the experimental chicks in the other groups were inoculated with IBDV by nasal intubation and were continuously observed for 2 weeks,meanwhile,the survival rate was statistically analyzed. The IBDV load was detected for analyzing the distribution of virus in tissues by RT-qPCR. The results showed that the TCME could improve the survival rate of chicks,the survival rate of chicks in groups of high,medium and low dose reached 86.67%,93.33%,70.00%,respectively. The spleen index and bursal index of chicks inoculated with IBDV in high dose group significantly decreased and thymus index and bursal index of chicks in medium dose group significantly decreased.The IBDV load of spleen,thymus and bursa in high and medium dose group were significantly lower than that in model group,the spleen and thymus were significantly lower than that in low-dose group,bursa were significantly lower than that in low-dose group. These results indicated that TCME could improve the survival rate of chicks and promote IBDV positive to negative,improve the chicks’spleen,thymus and bursal organ in its lower virus load,and the effect of medium dose is superior to the other doses. |
