| 水环境中Ni2+对鲤鱼鳃和肝脏的组织损伤 |
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| 引用本文：陈代宇，杨 飞，陈德芳，耿 毅.水环境中Ni2+对鲤鱼鳃和肝脏的组织损伤[J].西北农业学报,2020,30(2):175~181 |
| DOI:10.7606/j.issn.1004-1389.2020.02.002 |
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| 基金项目:四川省重点研发项目（2018NZ0007）；四川省淡水鱼产业技术体系创新团队建设项目（No.2017SICAD003）。 |
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| 中文摘要:为研究水环境中Ni2+对鲤鱼鳃和肝脏的组织损伤，将鲤鱼（Cyprinus carpio）暴露在Ni2+浓度为0、0.55、0.62、0.72、0.83、0.96、1.09、1.25 mmol/L的水环境中进行急性毒性试验，并通过组织切片观察鳃和肝脏的组织病理变化。结果显示，Ni2+对鲤鱼的96 h半数致死浓度（LC50）为0.72 mmol/L，安全浓度（SC）为 0.007 2 mmol/L。高浓度Ni2+（1.25 mmol/L）试验组鲤鱼的鳃小片呼吸上皮细胞坏死、脱落，肝血窦淤血，肝细胞核固缩；中低浓度Ni2+（0.72～1.09 mmol/L）试验组鲤鱼的鳃小片呼吸上皮水肿浮离、细胞增生，血窦充血，肝细胞胞浆空泡化。表明水环境中的Ni2+浓度高于0.007 2 mmol/L时会对鲤鱼的鳃和肝脏造成急性毒性，导致鳃和肝脏出现明显的组织病理变化。 |
| 中文关键词:鲤鱼 重金属 镍 急性毒性 病理 |
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| Histopathology of Gill and Liver of Cyprinus carpio Exposed to Ni2+in Aquatic Environment |
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| Abstract:In order to investigate the pathological effect of Ni2+ on the gills and liver of common carp (Cyprinus carpio),the fish were exposed to different Ni2+ concentration with 0, 0.55 mmol/L, 0.62 mmol/L, 0.72 mmol/L, 0.83 mmol/L, 0.96 mmol/L, 1.09 mmol/L and 1.25 mmol/L for acute toxicity experiment.The various histopathological changes of the gills and liver were observed through tissue sections.The results showed that the median lethal concentration(LC50) was 0.72 mmol/L in 96 h and the safe concentration (SC) was 0.007 2 mmol/L.In high concentration group (1.25 mmol/L), the respiratoryepithelial cell necrosis and shedding of epithelial cells in secondary gill lamella, hepatic sinus congestion and nuclear condensation of hepatocyte were observed. In medium and low concentration (0.72 mmol/L-1.09 mmol/L), the respiratoryepithelial cells edema and proliferation in the secondary gill lamella, and hepatocyte vacuolation. This study confirmed that Ni2+ concentration higher than 0.007 2 mmol/L in aquatic environment would cause acute toxicity and pathological changes to the gills and liver of carp. |
| keywords:Carp Heavy metal Nickel Acute toxicity Histopathology |
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