| 异迟眼蕈蚊对溴氰菊酯的抗性风险及解毒酶活力分析 |
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| 引用本文：沈登荣,何 超,袁盛勇,田学军,李 珣,张宏瑞.异迟眼蕈蚊对溴氰菊酯的抗性风险及解毒酶活力分析[J].西北农业学报,2019,28(7):1195~1202 |
| DOI:10.7606/j.issn.1004-1389.2019.07.020 |
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| 基金项目:云南省地方本科高校联合基金（2017FH001-038）；云南省教育厅科研基金（2013Z148）；红河学院植物保护硕士点建设项目。 |
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| 中文摘要:为了探讨异迟眼蕈蚊对溴氰菊酯的抗性风险和抗性生化机理，在室内采用溴氰菊酯对异迟眼蕈蚊进行抗性选育，对其抗性现实遗传力和解毒酶活力进行分析。结果表明：采用个体选育法和群体选育法分别对敏感品系和抗性品系进行选育，抗性选育24代后，抗性倍数达到11.36倍，现实遗传力h为 0.077 1。当致死率为80%~90%时，预计异迟眼蕈蚊对溴氰菊酯的抗性增长10倍需要11~14代。增效剂对溴氰菊酯的增效结果表明，抗性品系中增效醚和磷酸三苯酯对溴氰菊酯的增效作用显著（P<0.05）。解毒酶活性分析表明，随着抗性选育代数的增加，多功能氧化酶O-脱甲基和羧酸酯酶的活性显著增加（P<0.05）。说明异迟眼蕈蚊对溴氰菊酯的抗性风险较小，但在食用菌生产中也要尽量降低溴氰菊酯的使用浓度；多功能氧化酶和羧酸酯酶活性的增强是异迟眼蕈蚊对溴氰菊酯产生代谢抗性的主要原因。 |
| 中文关键词:异迟眼蕈蚊 溴氰菊酯 抗性风险 解毒酶 |
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| Resistance Risk and Activity of Detoxification Enzyme to Deltamethrin in Bradysia difformis |
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| Abstract:In order to explore the resistance risk and biochemical mechanism of deltamethrin in Bradysia difformis,selection of B.difformis resistance to deltamethrin was performed in the laboratory,and realized heritability and detoxification enzyme activity of deltamethrin in B.difformis were analyzed. The results showed that the susceptible strain and resistant strain were selected by individual selection and group selection respectively,After 24 generations of resistant selection,a resistant strain with a resistance ratio of 11.36-fold was obtained,and its realized heritability (h) was 0.077 1.If the lethallty rate was 80%-90%,B.difformis was expected to obtain 10-fold resistance to deltamethrin after resistant selection of 11 to 14 generations. The synergistic effect of synergist to deltamethrin showed that piperonyl butoxide and triphenyl phosphate had significant synergistic effect on deltamethrin in resistant strain (P<0.05). The activity analysis of detoxifying enzyme showed that with the increase of selective generation,the activity of O-demethylation of multi-functional oxidase and carboxylate enzyme was significantly increased (P<0.05). The results indicated that B.difformis may has low risk resistance to deltamethrin,it was necessary to reduce the concentration of deltamethrin in edible fungus production,and the enhanced activity of multifunctional oxidase and carboxylate enzyme may be the main cause of metabolic resistance to deltamethrin in B.difformis. |
| keywords:Bradysia difformis Deltamethrin Resistance risk Detoxification enzyme |
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