| Abstract:This study is to investigate the effects of glycyrrihizic stem and leaf supplemental levels on creatinine,thiamine,cholesterol,fatty acids and amino acids in Altay mutton sheep,to explore the suitable glycyrrihizic stem and leaf supplemental levels for fattening period of Altay sheep.60 Altay mutton sheep with the age of 6 months and an average body weight of (40±2.5) kg were randomly assigned to 6 groups with 10 sheep each group.Sheep in groupⅠ(control group) were fed a basal diet,while the sheep in group Ⅱ to Ⅵ (experimental groups) were fed with experimental diets supplemented with 10.01%,14.95%,20.41%,29.98%,60.11% glycyrrihizic stem and leaf on the basis of the basal diet,respectively.The experimental period was 60 days.The results showed that the thiamine quality fraction in longissimus dorsi of altay sheep of group Ⅵ was significantly lower than that of control group (P<0.05),there were no significant differences between other experimental groups and control group (P>0.05).Though glycyrrihizic stem and leaf supplemental levels had no significant influences on inosinic acid and cholesterol quality fraction in longissimus dorsi (P>0.05),inosinic acid quality fraction in group Ⅳ was the highest,and 20.67% higher than that of control group,and cholesterol quality fraction in group Ⅳ was the lowest and 45.33% lower than that of control group.Compared with control group,eicosendioic acid quality fraction in longissimus dorsi in group Ⅲ decreased significantly(P<0.01),docosahexaenoic acid and docosanoic acid quality fraction decreased significantly(P<0.01).While there were no significant differences among other experimental groups (P>0.05).From the view of stearic acid,the main saturated fatty acid in longissimus dorsi,the degree that decreased in group Ⅳ was the largest and reduced by 10.55%.Decision from oleic acid,the main unsaturated fatty acid,the extent of increase in group Ⅲ was the largest and increased by 5.54%.From the view of linolenic acid,the essential fatty acid,which increased only in group Ⅳ.The quality fraction of total unsaturated fatty acid in group Ⅴ was lower than other groups and the quality fraction of total unsaturated fatty acid was higher than other groups.The methionine levels in longissimus dorsi of group Ⅵ decreased significantly(P<0.01).Tyrosine levels in longissimus dorsi of group Ⅲ decreased significantly(P<0.05).Serine,alanine,valine,isoleucine,leucine,aspartic acid and total amino acids of longissimus dorsi in group Ⅳ were significantly higher than that of group Ⅵ (P<0.05),while there were no significant differences among control group and other experimental groups (P>0.05).In addition,the quality fraction of glutamic acid,aspartic acid and alanine of longissimus dorsi in group Ⅳ were higher than other groups.It is concluded that group Ⅳ is helpful to improve the levels of inosine acid,cholesterol,flavor amino acids and essential fatty acids in longissimus dorsi.Group Ⅳ and Ⅴ are helpful to improve the quality fraction of unsaturated fatty acids and essential fatty acids in longissimus dorsi.Comprehensively,20.41% glycyrrihizic stem and leaf supplemental level is best,29.98% glycyrrihizic stem and leaf supplemental level is next to it. |
