| 甜樱桃栽培种指纹图谱构建及遗传多样性分析 |
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| 引用本文：王丹丹，付化瑞，张彦文.甜樱桃栽培种指纹图谱构建及遗传多样性分析[J].西北农业学报,2017,(12):1813~1820 |
| DOI:10.7606/j.issn.1004-1389.2017.12.010 |
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| 基金项目:国家自然科学基金（31370400）；丹东市2016科技攻关（2016KJ001）；辽东学院青年基金（2015QN006）。 |
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| 中文摘要:为了对甜樱桃品种进行分子鉴别及分析不同品种间的亲缘关系，以叶片直接进行PCR扩增，采用EST-SSR分子标记技术构建生产上常用的40个甜樱桃栽培品种的数字指纹图谱和模式指纹图谱数据库（Cluster Project）并进行遗传多样性分析。采用24对甜樱桃核心引物对所有品种基因组进行扩增，共检测到129个等位位点，包括118个多态性位点，多态性比率为91.2%。每对引物扩增出的等位位点数3～10个，每对引物平均扩增5.38个基因型，扩增条带大小介于100～500 bp，多态信息含量（PIC）为0.654，基因流（Nm）为1.402，平均杂合率为0.495。8对引物，即PA17、PA32、PA51、PA54、PA73、PA99、PA101和PA202可作为指纹图谱构建的首选引物，利用其中的PA17、PA51、PA99、PA101和PA202以引物组合方式构建指纹图谱可区分40个品种；聚类分析表明在遗传距离0.71处40个品种被分为5类，聚类结果与品种的特性及成熟期具有较高的一致性。 |
| 中文关键词:甜樱桃 EST-SSR标记 指纹图谱 遗传多样性 聚类分析 |
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| Establishment of DNA Fingerprinting and Analysis of Genetic Diversity among Prunus aviun Cultivars |
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| Abstract:In order to identify the cultivars of Prunus aviun and analyze the genetic relationship among different cultivars at a molecular level, we constructed the digital fingerprint and model fingerprint（ClusterProject）for 40 common Prunus aviun cultivars using EST-SSR(Expressed Sequence Tag-Simple Sequence Repeats) molecular markers. The 24 pairs of primers amplified a total of 129 alleles including 118 polymorphic alleles with a polymorphism rate of 91.2%. Each pairs of primers can amplify 3-10 alleles, with an average of 5.38 alleles per locus. The length of amplified products was 100-500 bp, with the mean value of PIC(Polymorphism information content) being 0.654,Nm(Gene flow) being 1.402, and the mean value of Ho(Heterozygosity) being 0.495. Among the 24 primers, 8 primers can be used as a preferred choice for fingerprinting, namely PA17, PA32, PA51, PA54，PA73, PA99，PA101 and PA202, in which 5 primers(PA17, PA51, PA99, PA101 and PA202) can be employed in combination to identify the 40 cultivars. Cluster analysis showed that the 40 cultivars can be divided into five groups at a genetic distance of 0.71; the genetic relationship as indicated by the cluster completely reflected the characteristics and the maturation stage of the cultivars. |
| keywords:Prunus aviun EST-SSR markers Fingerprinting Genetic diversity Cluster analysis |
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