| 茶树‘紫娟’肉桂酰辅酶A还原酶基因（CCR）克隆及序列分析 |
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| 引用本文：陈林波，宋维希，李晓霞，夏丽飞，周 萌，梁名志，焉文光.茶树‘紫娟’肉桂酰辅酶A还原酶基因（CCR）克隆及序列分析[J].西北农业学报,2016,25(1):80~85 |
| DOI:10.7606/j.issn.1004-1389.2016.01.011 |
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| 基金项目:国家自然科学基金(31560220，31460216)；云南省农业科学院专项基金（YAAS2013JC001，YAAS2014JC012）；茶树生物学与资源利用国家重点实验室开放基金（LTBB20140103）。 |
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| 中文摘要:通过克隆茶树肉桂酰辅酶A还原酶基因（CCR）的cDNA序列，为研究其蛋白结构和功能奠定基础。对利用cDNA AFLP技术获得的‘紫娟’茶树成熟叶片上调的差异表达片段TDF，设计特异引物，利用RACE末端扩增技术，分别扩增出5′端和3′端目的片段，测序后进行拼接获得茶树肉桂酰辅酶A还原酶基因（CCR）cDNA全长序列，并进行序列分析。结果表明：克隆的茶树CsCCR基因cDNA全长1 259 bp (GenBank登录号为KJ995737)，其中开放阅读框957 bp。同源比对发现，与蓖麻、丹参、草莓、番茄的CCR蛋白同源性分别为67%、68%、69%和70%。 |
| 中文关键词:茶树 肉桂酰辅酶A还原酶 基因克隆 序列分析 |
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| Cloning and Sequence Analysis of Cinnamoyl CoA Reductase Gene from Tea Plant［Camellia sinensis (L.)O.Kuntz］ |
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| Abstract:In order to lay a foundation for the structure and function studies of CsCCR protein, CsCCR cDNA of tea plant was cloned. The cDNA AFLP was applied to up regulated transcript derived fragment (TDF) from mature leaves of tea plant (Camellia sinensis var.assamica, cultivar ‘Zijuan’). TDF was used to design specific primers, and then cloning the 5′ and 3′ end sequence by RACE, and fragment was assembled to get completely cDNA. Finally, CsCCR cDNA sequence was analyzed. The results show that the cDNA of CsCCR was 1 259 bp in length (GenBank accession No.KJ995737), and contains an open reading frame (ORF) of 957 bp and encoding 319 amino acids residues. It was found that the amino acid sequence of CsCCR had 67%, 68%, 69% and 70% homology with Ricinus communis, Salvia miltiorrhiza, Fragaria vesca, and Solanum lycopersicum, respectivly. |
| keywords:Camellia sinensis Cinnamoyl CoA reductase(CCR) Gene cloning Sequence analysis |
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