| 郭海超,杨建华,李志浩,缐旭林,李彩龙,高彦龙,王延秀.茉莉酸甲酯响应盐碱胁迫下苹果砧木M26的生理机制[J].干旱地区农业研究,2024,(1):159~168 |
| 茉莉酸甲酯响应盐碱胁迫下苹果砧木M26的生理机制 |
| Physiological mechanisms of methyl jasmonate in apple rootstock M26’s response to saline\|alkali stress |
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| DOI：10.7606/j.issn.1000-7601.2024.01.16 |
| 中文关键词: 苹果砧木M26 茉莉酸甲酯 盐碱胁迫 生理效应 |
| 英文关键词:apple rootstock M26 methyl jasmonate saline\|alkali stress physiological effects |
| 基金项目:国家自然科学基金（31960581） |
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| 摘要点击次数: 297 |
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| 中文摘要: |
| 为探索外源茉莉酸甲酯（MeJA）处理后苹果砧木对盐碱胁迫的响应特性，以苹果砧木M26为试验材料，设置CK1（清水浇灌)、CK2（盐碱胁迫：200 mmol·L-1NaCl∶NaHCO3=1∶1）、T1（盐碱胁迫+0.05 μmol·L-1MeJA）、T2（盐碱胁迫+0.5 μmol·L-1MeJA）、T3（盐碱胁迫+5 μmol·L-1MeJA）、T4（盐碱胁迫+50 μmol·L-1MeJA）、T5（盐碱胁迫+500 μmol·L-1MeJA）共7个处理，观察并测定各处理下苹果砧木生长的表型、光合色素以及渗透调节物质含量，并对其进行相关性和主成分分析。结果表明：与CK2相比，喷施MeJA处理幼苗叶片边缘黄化未扩大，叶绿素a（Chl a）含量、叶绿素b（Chl b）含量、叶绿素a+b（Chl a+b）含量、净光合速率（Pn）、可溶性糖（SS）含量、可溶性蛋白（SP）含量、超氧化物歧化酶（SOD）活性、脯氨酸（Pro）含量、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性均有所升高，其中T4升幅最大，各指标平均增幅范围为12.77%~69.80%；喷施MeJA处理的叶片电导率（REC）、超氧阴离子（O2）含量、丙二醛（MDA）含量相比CK1有所升高，T1、T2、T3、T5处理各指标升高幅度范围为30.5%~109.2%，其中T4增幅最小，为18.72%~45.02%。相关性分析表明，Chl a与Chl b、Chl a+b含量呈极显著正相关关系，与MDA、O2含量及REC呈极显著负相关关系，与SP、H2O2含量呈显著负相关关系。对17个指标进行主成分分析，3个主成分累积方差贡献率达到95.25%；依据主成分进行综合排序，外源MeJA对盐碱胁迫下M26幼苗叶片缓解能力表现为T4>T5>T3>T2>T1。综上，在200 mmol·L-1浓度盐碱胁迫下，施用50 μmol·L-1的外源茉莉酸甲酯对苹果砧木生理特性的调节效果最佳。 |
| 英文摘要: |
| To explore the response characteristics of apple rootstock to salt\|alkaline stress following treatment with exogenous Methyl Jasmonate (MeJA), the apple rootstock M26 was used as the experimental material. Seven treatment groups were established, including CK1 (control with normal watering), CK2 (salt\|alkaline stress: 200 mmol·L-1 NaCl∶NaHCO3 in 1∶1 ratio), T1 (salt\|alkaline stress + 0.05 μmol·L-1 MeJA), T2 (salt\|alkaline stress + 0.5 μmol·L-1 MeJA), T3 (salt\|alkaline stress + 5 μmol·L-1 MeJA), T4 (salt\|alkaline stress + 50 μmol·L-1 MeJA), and T5 (salt\|alkaline stress + 500 μmol·L-1 MeJA). Phenotypic growth, photosynthetic pigments, and osmotic regulatory substance contents of the apple rootstock were observed and measured under each treatment. Correlation and principal component analyses were conducted. The results indicated that compared to CK2, MeJA treatments on seedling leaves did not significantly expand leaf edge yellowing. It led to increased levels of chlorophyll a (Chl a) content, chlorophyll b (Chl b) content, total chlorophyll (Chl a+b) content, net photosynthetic rate (Pn), soluble sugars (SS) content, soluble proteins (SP) content, superoxide dismutase (SOD) activity, proline (Pro) content, peroxidase (POD) activity, and catalase (CAT) activity. Among these, T4 exhibited the highest increase, with an average range of 12.77% to 69.80%. MeJA treatment resulted in elevated leaf electrical conductivity (REC), superoxide anion (O2) content, and malondialdehyde (MDA) concentration compared to CK1. The increases in T1, T2, T3, and T5 ranged from 30.5% to 109.2%, with T4 showing the smallest increase at 18.72% to 45.02%. Correlation analysis revealed a highly significant positive correlation between Chl a and Chl b as well as Chl a+b content, and a highly significant negative correlation with MDA, O2 content, and REC. A significant negative correlation was observed with SP and H2O2 content. Principal component analysis of 17 indicators showed that three principal components accounted for 95.25% of the cumulative variance. Based on the principal components, the comprehensive ranking of MeJA’s alleviating effect on M26 seedling leaves under salt\|alkaline stress was T4>T5>T3>T2>T1. In conclusion, under 200 mmol·L-1 salt\|alkaline stress, application of 50 μmol·L-1 exogenous methyl jasmonate (MeJA) had the most effective regulatory impact on the physiological characteristics of apple rootstock M26. |
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