耐盐苯酚降解菌Staphylococcus sp.的分离及降解特性
Isolation and biodegradation characteristics of a halophilic phenol-degrading strain Staphylococcus sp.
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摘要: 从巴丹吉林沙漠盐湖表层沉积物中筛选到一株高效耐盐苯酚降解菌CL.测定了菌株CL的生理生化指标、16S rRNA基因序列，通过动力学模型探究了该菌株的生长和苯酚降解特性，同时考察了固定化对其耐受及降解苯酚能力的影响.结果表明，菌株CL属于葡萄球菌属（Staphylococcus sp.），在温度30℃、pH 7.0-8.0、盐度0-10%和苯酚浓度100-200 mg·L-1条件下，该菌株能高效降解苯酚，其降解率均在85%以上.菌株CL对不同浓度苯酚的降解符合Haldane模型，其最大比降解速率和抑制常数分别为0.32 h-1和351.70 mg·L-1，同时该菌株在不同盐度下对苯酚的降解符合Ghose and Tyagi模型.固定化可以明显增加菌株CL对苯酚的降解和耐受能力.菌株CL在高盐环境下能够高效降解苯酚，具有生物处理高盐含酚废水的潜力.
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关键词:
- Staphylococcus sp. /
- 苯酚 /
- 耐盐菌 /
- 动力学 /
- 固定化
Abstract: The haloph ilic and high-efficient phenol-degrading strain CL was screened from the surface sediments of Saline Lake in Badain Jilin desert. Physiological-biochemical indicators and 16S rRNA gene sequence were detected and kinetics models were used to investigate the characteristics of growth and phenol degradation, and the effect of immobilization on the phenol tolerance and degradation of strain CL. The results showed that the strain CL was identified as Staphylococcus sp., and it could efficiently degrade phenol (degradation rate of above 85%) with incubation at 30℃, pH 7.0-8.0, salinity 0-10% and phenol concentration 100-200 mg·L-1. The phenol degradation with different concentration by strain CL followed Haldane model (maximum specific degradation rate=0.32 h-1, inhibition constant=351.70 mg·L-1). The phenol degradation with different salinity by strain CL followed Ghose and Tyagi model. The immobilization could significantly increase the phenol tolerance and degradation ability of strain CL. The halophilic and high-efficient phenol-degrading characteristics of strain CL provide a new potential to be utilized in the high-salt phenolic wastewater.-
Key words:
- Staphylococcus sp. /
- phenol /
- halophile /
- kinetics /
- immobilization
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