强化微生物修复石油污染海洋的研究进展

刘雅娟, 赵建国, 武彦芳. 强化微生物修复石油污染海洋的研究进展[J]. 环境化学, 2019, (12): 2826-2835. doi: 10.7524/j.issn.0254-6108.2019040906
引用本文: 刘雅娟, 赵建国, 武彦芳.

强化微生物修复石油污染海洋的研究进展

[J]. 环境化学, 2019, (12): 2826-2835. doi: 10.7524/j.issn.0254-6108.2019040906
LIU Yajuan, ZHAO Jianguo, WU Yanfang. Enhanced microbial remediation of petroleum-pollutioned environment marine[J]. Environmental Chemistry, 2019, (12): 2826-2835. doi: 10.7524/j.issn.0254-6108.2019040906
Citation: LIU Yajuan, ZHAO Jianguo, WU Yanfang.

Enhanced microbial remediation of petroleum-pollutioned environment marine

[J]. Environmental Chemistry, 2019, (12): 2826-2835. doi: 10.7524/j.issn.0254-6108.2019040906

强化微生物修复石油污染海洋的研究进展

    通讯作者: 刘雅娟, E-mail: dtdxliuyajuan@139.com 赵建国, E-mail: jgzhaoshi@163.com
  • 基金项目:

    山西省回国留学人员资助项目(2017-108)和大同市重点研发计划(2018020)资助.

Enhanced microbial remediation of petroleum-pollutioned environment marine

    Corresponding authors: LIU Yajuan, dtdxliuyajuan@139.com ;  ZHAO Jianguo, jgzhaoshi@163.com
  • Fund Project: Supported by Shanxi Scholarship Council of China(2017-108)and Datong City Key Research and Development Projects (2018020).
  • 摘要:

    海洋石油污染使石油烃(Petroleum hydrocarbons,PHCs)成为海洋重要有机污染物.快速有效清除石油污染物是当前急需解决的海洋环境问题之一.利用石油烃降解菌(Petroleum hydrocarbon-degrading bacteria,PHDB)进行的原位微生物修复是一种对环境友好且较经济的清除石油污染场所和管理海洋表面溢油的有效技术.本文综述了石油烃降解菌的特性和对环境的适应性.重点分析探讨了引入外源微生物,投加生物表面活性剂、添加营养物质和增加溶解氧含量、采用微生物固定化技术等方法强化原位微生物修复石油污染海洋的机制和研究进展.展望了海洋石油污染微生物修复领域的研究前景.

  • 加载中
  • [1] 李丽, 张利平, 张元亮. 石油烃类化合物降解菌的研究概况[J]. 微生物学通报, 2001, 28(5):89-93.

    Li l, ZHANG L P, ZHANG Y L. Proceeding of degrading microorganisms of oil hydrocarbons[J]. Microbiology, 2001, 28(5):89-93(in Chinese).

    [2] QIAO N, SHAO Z. Isolation and characterization of a novel biosurfactant produced by hydrocarbon-degrading bacterium Alcanivorax dieselolei B-5[J]. Journal of Applied Microbiology, 2010, 108(4):1027-1216.
    [3] GOLYSHIN P N, SANTOS V A M D, KAISER O. Genome sequence completed of Alcanivorax borkumensis, a hydrocarbon-degrading bacterium that plays a global role in oil removal from marine systems[J]. Journal of Biotechnololy, 2003, 106(2):215-220.
    [4] 李贵珍, 赖其良, 闫培生, 等. 海洋石油污染及其微生物修复研究进展[J]. 生物技术进展, 2015, 5(3):164-169.

    LI G Z, LAI Q L, YAN P S, et al. Advance on marine petroleum pollution and microbial remediation[J]. Current Biotechnology, 2015, 5(3):164-169(in Chinese).

    [5] CUI Z, LAI Q, DONG C, et al. Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic ridge[J]. Environment Microbiology, 2008, 10(8):2138-2149.
    [6] KASAI Y, KISHIRA H, HARAYAMA S. Bacteria belonging to the genus Cycloclasticus play a primary role in the degradation of aromatic hydrocarbons released in a marine environment[J]. Applied and Environmental Microbiology, 2002, 68(11):5625-5633.
    [7] WANG B, LAI Q, CUI Z, et al. A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1[J]. Environment Microbiology, 2008, 10(8):1948-1963.
    [8] ISAAC P, SÁNCHEZ L A, BOURGUIGNON N, et al. Indigenous PAH-degrading bacteria from oil-polluted sediments in Caleta Cordova, Patagonia Argentina[J]. International Biodeterioration & Biodegradation, 2013, 82(7):207-214.
    [9] KACZOREK E, SAŁEK K, GUZIK U, et al. The impact of long-term contact of Achromobacter sp. 4(2010) with diesel oil-changes in biodegradation, surface properties and hexadecane monooxygenase activity[J]. International Biodeterioration & Biodegradation, 2013, 78(3):7-16.
    [10] BACOSA H, SUTO K, INOUE C. Preferential degradation of aromatic hydrocarbons in kerosene by a microbial consortium[J]. International Biodeterioration & Biodegradation, 2010, 64(2):702-710.
    [11] 王兴彪. 迪茨氏菌高效降解石油烃机理及在环境管理和石油工业中的应用研究[D]. 北京:中国科学院研究生院, 2012. WANG X B. The efficient degradation mechanism of petroleum hydrocarbons by Dietzia sp. and applied research in environmental management and petroleum industry[D]. Beijing:University of Chinese Academy of Science, 2012(in Chinese).
    [12] 邸富荣, 宋东辉, 刘凤路, 等. 分离海洋不动杆菌及其对石油烃降解性能研究[J]. 海洋环境科学, 2017, 36(6):898-904.

    DI F R, SONG D H, LIU F L, et al. Isolation of marine Acinetobacter and its characteristics of petroleum hydrocarbon degradation[J]. Marine Environmental Science, 2017, 36(6):898-904(in Chinese).

    [13] SEO J-S, KEUM Y-S, LI Q X. Bacterial degradation of aromatic compounds[J]. International Journal of Environmental Research and Public Health, 2009, 6(1):278-309.
    [14] ILORI M O, AMOBI C J, ODOCHA A C. Factors affecting biosurfactant production by oil degrading Aeromonas spp. isolated from a tropical environment[J]. Chemosphere, 2005, 61(7):985-992.
    [15] ALVAREZ H M. Relationship between β-oxidation pathway and the hydrocarbon-degrading profile in actinomycetes bacteria[J]. International Biodeterioration & Biodegradation, 2003, 52(1):35-42.
    [16] KIYOHARA H, NAGAO K, KOUNO K, et al. Phenanthrene-degrading phenotype of Alcaligenes faecalis AFK2[J]. Applied Environmental Microbial, 1982, 43(2):458-461.
    [17] SATHISHKUMAR M, BINUPRIYA A R, BAIK S-H, et al. Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas[J]. Clean Soil Air Water, 2008, 36(1):92-96.
    [18] ZHONG Y, LUAN T, LIN L, et al. Production of metabolites in the biodegradation of phenanthrene, fluoranthene and pyrene by the mixed culture of Mycobacterium sp. and Sphingomonas sp.[J]. Bioresource Technology, 2011, 102(3):2965-2972.
    [19] 沈伟航, 朱能武, 尹富华, 等. 微生物修复石油污染土壤的生态毒性指示[J]. 生态环境学报, 2015, 24(9):1560-1569.

    SHEN W H, ZHU N W, YING F H, et al. Study on combined bioindicators in ecotoxicity monitoring of oil-contaminated soil during bioremediation[J]. Ecology and Environmnet, 2015, 24(9):1560-1569(in Chinese).

    [20] VARJANI S J, UPASANI V N. Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514[J]. Bioresource Technology, 2016, 222(24):195-201.
    [21] 马永松, 李琋, 李珍珍, 等. 一株镍抗性和石油烃降解菌的分离鉴定及其生物学特性[J]. 生物技术通报, 2017, 33(10):169-177.

    MA Y S, LI X, LI Z Z, et al. Isolation and identification of a nickel-resistant and petroleum hydrocarbon degrading strain and its biological characteristics[J]. Biotechnology Bulletin, 2017, 33(10):169-177(in Chinese).

    [22] ZHANG H, TANG J, WANG L, et al. A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar[J]. Journal of Environmental Sciences, 2016, 47(9):7-13.
    [23] 夏瑛铭, 张朝晖, 王亮, 等. 外加碳源对红球菌IcdP1降解荧蒽的特性研究[J]. 环境科学与技术, 2017, 40(9):14-19.

    XIA Y M, ZHANG Z H, WANG L, et al. Effects of external carbon sources on degradation of fluoranthene by Rhodococcus sp. Icd P1[J]. Environmental Science and Technology, 2017, 40(9):14-19(in Chinese).

    [24] ZHAO Z, SUN J, ZHANG R, et al. Binariimonas pacifica gen. nov., sp. nov., a Novel marine bacterium of family Sphingomonadaceae isolated from East Pacific Ocean Surface Seawater[J]. Current Microbiology, 2016, 72(3):276-281.
    [25] 刘仁菊, 董纯明, 李登峰, 等. 海洋石油降解菌深海弯曲菌的种类多样性及地理分布特征分析[J]. 应用海洋学学报, 2017, 36(4):565-574.

    LIU R J, DONG C M, LI D F, et al. Species diversity and biogeographic distribution of marine oil-degrading bacteria Thalassolituus spp.[J]. Journal of Oceanography in Taiwan Strait, 2017, 36(4):565-574(in Chinese).

    [26] 长安大学. 一种海洋石油降解菌、菌剂及其应用:中国, 201611242621.6[P]. 2017-05-10.

    Chang'an University. A marine petroleum-degrading bacterium and its application:China, 201611242621.6[P]. 2017-05-10(in Chinese).

    [27] 国家海洋局第三海洋研究所, 中国大洋矿产资源研究开发协会. 一株海洋石油降解菌、发酵所得化合物及其用途:中国, 201410354363.5[P]. 2015-04-08.

    Third Institute of Oceanography, Ministry of Natural Resources, China Ocean Mineral Resources R&D Association. A marine oil-degrading bacterium, fermentation compounds and their applications:China, 201410354363.5[P]. 2015-04-08(in Chinese).

    [28] 长江大学. 一株石油芳香烃降解菌及其应用:中国, 201810004789.6[P]. 2018-04-10.

    Yangtze University. An oil aromatic hydrocarbon-decomposition bacterium and its application:China, 201810004789.6[P]. 2018-04-10(in Chinese).

    [29] 青岛双瑞海洋环境工程股份有限公司. 石油降解菌Oil 2-3、石油降解菌Oil 2-3分离纯化方法及其应用:中国, 201710003626.1[P]. 2017-05-31.

    Qingdao Sunrui Marine Environment Engineering Co., LTD.. Oil-degrading bacterium Oil 2-3, isolation and purification method and application of Oil 2-3:China, 201710003626.1[P]. 2017-05-31(in Chinese).

    [30] 大连海事大学. 具有低温降解多环芳烃功能的交替假单胞菌及其应用:中国, 201610290987.4[P]. 2016-08-31.

    Dalian Maritime University. Alternative Pseudomonas that can degrade of polycyclic aromatic hydrocarbons at low temperature and its application:China, 201610290987.4[P]. 2016-08-31(in Chinese).

    [31] 大连海事大学. 一株具有低温降解石油功能的红球菌及其应用:中国, 201610289660.5[P]. 2016-08-31.

    Dalian Maritime University. A Rhodococcus sp. that can degrade of oil at low temperature and its application:China, 201610289660.5[P]. 2016-08-31(in Chinese).

    [32] 大连海事大学. 一株具有低温降解石油功能的交替假单胞菌及其应用:中国, 201610290966.2[P]. 2016-08-31.

    Dalian Maritime University. A Pseudoalteromonas sp. with degradation of oil at low temperature and its application:China, 201610290966.2[P]. 2016-08-31(in Chinese).

    [33] 中国科学院烟台海岸带研究所. 一种石油降解菌株及分离方法、石油降解菌剂及其制备方法与应用:中国, 201510617222.2[P]. 2016-04-20.

    Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences. A petroleum degradation strain and its separation method, a petroleum degradation microorganism and its preparation method and application:China, 201510617222.2[P]. 2016-04-20(in Chinese).

    [34] 中国科学院烟台海岸带研究所. 一种石油烃降解菌株、石油烃降解菌剂及其制备方法与应用:中国, 201610489513.2[P]. 2016-11-16.

    Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences. A petroleum hydrocarbon degradation strain, petroleum hydrocarbon degradation microorganism and its preparation method and application:China, 201610489513.2[P]. 2016-11-16(in Chinese).

    [35] 国家海洋局第三海洋研究所. 一株石油烃降解菌DL8-7及其应用:中国, 201410247866.2[P]. 2014-08-27.

    Third Institute of Oceanography, Ministry of Natural Resources. DL8-7, a petroleum hydrocarbon degradation bacterium and its application:China, 201410247866.2[P]. 2014-08-27(in Chinese).

    [36] 国家海洋局第三海洋研究所. 一株石油烃降解菌DLFJ3-3及其应用:中国, 201410247867.7[P]. 2014-08-27.

    Third Institute of Oceanography, Ministry of Natural Resources. DLFJ3-3, a petroleum hydrocarbon degradation bacteria and its application:China, 201410247867.7[P]. 2014-08-27(in Chinese).

    [37] 国家海洋局第三海洋研究所. 一株石油烃降解菌DLFJ1-1及其降解基因与应用:中国, 201410248291.6[P]. 2014-08-27.

    Third Institute of Oceanography, Ministry of Natural Resources. DLFJ1-1, a petroleum hydrocarbon degradation bacterium and its degradation gene and application:China, 201410248291.6[P]. 2014-08-27(in Chinese).

    [38] 中国海洋石油总公司, 中海油能源发展股份有限公司, 中海石油环保服务(天津)有限公司. 一种应用于高盐环境下的石油降解复合菌及菌剂:中国, 201310144009.5[P]. 2013-08-07.

    China National Offshore Oil Corporation, CNOOC Energy Technology & Services Limited, CNOOC Environmental Services(Tianjin) Limited. An oil degradation compound bacterium and bacterial species used in high salt environment:China, 201310144009.5[P]. 2013-08-07(in Chinese).

    [39] 中国人民解放军海军医学研究所. 一株能降解海洋柴油污染物的细菌DW3:中国, 201110325954.6[P]. 2012-04-04.

    The Naval Medical I Research Institute. DW3, a bacterium that can degrade marine diesel pollutants:China, 201110325954.6[P]. 2012-04-04(in Chinese).

    [40] 中国海洋石油总公司, 中国海洋大学, 中海油能源发展股份有限公司, 等. 溢油污染海岸线生物修复用菌剂及制备方法:中国, 200910245097.1[P]. 2010-06-02.

    China National Offshore Oil Corporation, Ocean University of China, CNOOC Energy Technology & Services Limited, et al. Bacteria used for bioremediation of shoreline contaminated by oil spills and preparation methods:China, 200910245097.1[P]. 2010-06-02(in Chinese).

    [41] BOVIO E, GNAVI G, PRIGIONE V, et al. The culturable mycobiota of a Mediterranean marine site after an oil spill:isolation, identification and potential application in bioremediation[J]. Science of the Total Environment, 2017, 576(3):310-318.
    [42] ROY S, DIPAK HENS, BISWAS D, et al. Survey of petroleum-degrading bacteria in coastal waters of Sunderban Biosphere Reserve[J]. World Journal of Microbiology and Biotechnology, 2002, 18(6):575-581.
    [43] BRADDOCK J F, LINDSTROM J E, BROWN E J. Distribution of hydrocarbon-degrading microorganisms in sediments from Prince William Sound, Alaska, following the Exxon Valdez oil spill[J]. Marine Pollution Bulletin, 1995, 30(2):125-132.
    [44] EL-SHESHTAWY H S, KHALIL N M, AHMED W, et al. Monitoring of oil pollution at Gemsa Bay and bioremediation capacity of bacterial isolates with biosurfactants and nanoparticles[J]. Marine Pollution Bulletin, 2014, 87(1-2):191-200.
    [45] WANG H, WANG C, LIN M, et al. Phylogenetic diversity of bacterial communities associated with bioremediation of crude oil in microcosms[J]. International Biodeterioration & Biodegradation, 2013, 85(10):400-406.
    [46] 唐景春, 吕宏虹, 刘庆龙, 等. 石油烃污染及修复过程中的微生物分子生态学研究进展[J]. 微生物学通报, 2015, 42(5):944-955.

    TANG J C, LV H H, LIU Q L, et al. Recent review on the microbial molecular ecology during contamination and remediation of petroleum hydrocarbons[J]. Microbiology, 2015, 42(5):944-955(in Chinese).

    [47] 吴作军, 卢滇楠, 张敏莲, 等. 微生物分子生态学技术及其在石油污染土壤修复中的应用现状与展望[J].化工进展, 2010, 29(5):789-795.

    WU Z J, LU D N, ZHANG M L, et al. Progress in applications of microbiological molecular ecology in bioremediation of petroleum contaminated soil[J]. Chemical Industry and Engineering Progress, 2010, 29(5):789-795(in Chinese).

    [48] INCARDONA J P, SWARTS T L, EDMUNDS R C, et al. Exxon valdez to deepwater horizon:Comparable toxicity of both crude oils to fish early life stages[J]. Aquatic Toxicology, 2013, 142-143(10):303-316.
    [49] 陈丽华, 孙万虹, 李海玲, 等. 石油降解菌对石油烃中不同组分的降解及演化特征研究[J]. 环境科学学报, 2016, 36(1):124-133.

    CHEN L H, SUN W H, LI H L, et al. Biological degradation and transformation characteristics of different components in petroleum hydrocarbon by oil-degradation bacteria[J]. Acta Scientiae Circumstantiae, 2016, 36(1):124-133(in Chinese).

    [50] VARJANI S J, RANA D P, JAIN A K, et al. Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India[J]. International Biodeterioration & Biodegradation, 2015, 103(7):116-124.
    [51] ALISI C, MUSELLA R, TASSO F, et al. Bioremediation of diesel oil in a co-contaminated soil by bioaugmentation with a microbial formula tailored with native strains selected for heavy metals resistance[J]. Science of the Total Environment, 2009, 407(8):3024-3032.
    [52] 李伟光, 朱文芳, 吕炳南. 混合菌培养降解含有废水的研究[J]. 给水排水, 2003, 29(11):42-44.

    LI W G, ZHU W F, LV B N. Degradation of refinery wastewater by mixed culture bacteria[J]. Water & Wastewater Engineering, 2003, 29(11):42-44(in Chinese).

    [53] 毛丽华. 石油污染土壤生物通风堆肥修复研究[D]. 北京:中国地质大学, 2006. MAO L H. Study on compost restoration of bio-ventilation in petroleum contaminated soil[D]. Beijing:China University of Geosciences, 2006(in Chinese).
    [54] TZARKOVA E K, GROUDEVA V I. Bioremediation of petroleum-polluted soil by adsorptive immobilized Cornyebacterium sp. RB-96[J]. Biotechnology &Biotechnology Equipment, 2000, 14(1):72-75.
    [55] 郑天凌, 庄铁诚. 微生物在海洋污染环境中的生物修复作用[J]. 厦门大学学报:自然科学版, 2001, 40(2):524-534.

    ZHENG T L, ZHUANG T C. The role of microbes in bioremediation of marine polluted environmetn[J]. Journal of Xiamen University(Natural Science), 2001, 40(2):524-534(in Chinese).

    [56] 武金装. 柴油降解菌的筛选、降解条件优化及其降解机理研究[D]. 长沙:湖南大学, 2008. WU J Z. Screening, optimization of degradation condition and its degradation mechanism of diesel degradation bacteria[D]. Changsha, Hunan Univeraity, 2008(in Chinese).
    [57] PRANJAL B, BOLIN KUMAR K. Production and physico-chemical characterization of a biosurfactant produced by Pseudomonas aeruginosa OBP1 isolated from petroleum sludge[J]. Applied Biochemistry and Biotechnology, 2011, 16(8):1444-1460.
    [58] 腾小兰, 蒲万芬, 罗强, 等. 高分子生物表面活性剂性质的初步研究[J]. 江汉石油职工大学学报, 2008, 21(6):42-45.

    TENG X-L, FU W-F, LUO Q, et al. Preliminary studies on prosperties of high-molecular-weight biosurfactant[J]. Journal of Jianghan Petroleum University of Staff and Workers, 2008, 21(6):42-45(in Chinese).

    [59] 苏增建, 张玉秀, 李敏, 等. 热带海水中生物表面活性剂生产菌的筛选及特性研究[J]. 安全与环境学报, 2018, 18(4):1457-1463.

    SU Z J, ZHANG Y X, LI M, et al. Screening and characterization of the bacterium producing biosurfactant in the tropical seawater[J]. Journal of Safety and Environment, 2018, 18(4):1457-1463(in Chinese).

    [60] 刘真. 海洋烷烃降解菌的富集分离与多样性初步分析及其生物表面活性剂产生菌的筛选[D]. 厦门:厦门大学, 2008. LIU Z. The diversity of alkane degrading bacteria in the enrichments and the screening for the biosurfactant-producing bacteria[D]. Xiamen:Xiamen University, 2008(in Chinese).
    [61] HUGHES J B, BECKLES D M, WARD C H. Utilization of bioremediation processes for the treatment of PAH-contaminated sediments[J]. Journal of Industrial Microbiology & Biotechnology, 1997, 18(1):152-160.
    [62] DATTA P, TIWARI P, PANDEY L M. Isolation and characterization of biosurfactant producing and oil degrading bacillus subtilis MG495086 from formation water of Assam Oil Reservoir and its suitability for enhanced oil recovery[J]. Bioresource Technology, 2018, 270(25):439-448.
    [63] CHENGZHI Y, ZHUHONG L. Screening standards and geological bases for microbial enhanced oil recovery[J]. Petroleum Exploration and Development, 1997, 24(2):69-72.
    [64] 强婧, 尹华, 彭辉, 等.铜绿假单胞菌S6分泌的生物表面活性剂的特性[J]. 环境科学学报, 2009, 29(1):102-110.

    QIANG J, YIN H, PENG H, et al. Characteristics of a biosurfactant produced by Pseudomonas aeruginosa S6[J]. Acta Scientiae Circumstantiae, 2009, 29(1):102-110(in Chinese).

    [65] 雷依丰. 红平红球菌FF产海藻糖脂的特性及其在烃降解中的应用研究[D]. 西安:西安建筑科技大学, 2018. LEI Y F. Study on the characteristics and application of trehalose production by Rhizoma erythrococcus FF in hydrocarbon degradation[D]. Xi'an:Xi'an University of Architecture and Technology, 2018(in Chinese).
    [66] WANG C, LIU X, GUO J, et al. Biodegradation of marine oil spill residues using aboriginal bacterial consortium based on Penglai 19-3 oil spill accident, China[J]. Ecotoxicology and Environmental Safety, 2018, 159(13):20-27.
    [67] PI Y, BAO M, LIU Y, et al. The contribution of chemical dispersants and biosurfactants on crude oil biodegradation by Pseudomonas sp. LSH-7'[J]. Journal of Cleaner Production, 2017, 153:74-82.
    [68] PLANTE C J, COE K M, PLANTE R G. Isolation of surfactant-resistant bacteria from natural, surfactant-rich marine habitats[J]. Applied and Environmental Microbiology, 2008, 74(16):5093-5099.
    [69] LECHUGA M, FERNáNDEZ-SERRANO M, JURADO E, et al. Acute toxicity of anionic and non-ionic surfactants to aquatic organisms[J]. Ecotoxicology and Environmental Safety, 2016, 125:1-8.
    [70] BAMFORTH S M, SINGLETON I. Bioremediation of polycyclic aromatic hydrcabons:current kwnoledge and future direction[J]. Journal of Chemical Technology & Biotechnology, 2005, 80(7):723-736.
    [71] 张卉, 张笔威, 张妍, 等. 生物表面活性剂产生菌处理水体石油污染物的研究[J]. 安全与环境学报, 2015, 15(6):269-273.

    ZHANG H, ZHANG B W, ZHANG Y, et al. On the degradation of petroleum pollutants in the water by biosurfactant-producing bacteria[J]. Journal of Safety and Environment, 2015, 15(6):269-273(in Chinese).

    [72] CERQUEIRA V S, HOLLENBACH E B, MABONI F, et al. Biodegradation potential of oily sludge by pure and mixed bacterial cultures[J]. Bioresource Technology, 2011, 102(23):11003-11010.
    [73] STRAIGHT P D, WILLEY J M, ROBERTO K. Interactions between streptomyces coelicolor and bacillus subtilis:role of surfactants in raising aerial structures[J]. Journal of Bacteriology, 2006, 188(13):4918-4925.
    [74] 马斌斌, 马恒轶, 李少君, 等. 海洋柴油降解细菌的筛选鉴定及其生物表面活性剂特性[J]. 浙江农业科学, 2018, 59(3):400-404.

    MA B B, MA H Y, LI S J, et al. Screening and identification of marine diesel degradation bacteria and their biosurfactant characteristics[J]. Journal of Zhejiang Agricultural Sciences, 2018, 59(3):400-404(in Chinese).

    [75] WANG W, CAI B, SHAO Z. Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3[J]. Frontiers in Microbiology, 2014, 5:1-11.
    [76] 王冬梅, 赵连彪. 产生物表面活性剂石油降解菌的分离及特性研究[C]. 中国土壤学会土壤环境专业委员会第二十次会议暨农田土壤污染与修复研讨会, 中国安徽合肥, 2018. WANG D M, ZHAO L B. Isolation and characterization of biosurfactant-producing petroleum-degrading bacteria[C]. 20th Meeting of the specialized committee on soil environment of china soil society and seminar on soil pollution and rehabilitation of agricultural fields, Hefei Anhui Provice, 2018(in Chinese).
    [77] ROSENBERG E, ZUCKBERG A, RUBINOVITZ C, et al. Emulsifier of Arthrobacter RAG-1:Isolation and emmulsifying properties[J]. Applied and Environmental Microbiology, 1979, 37(3):402-408.
    [78] 华涛, 李胜男, 邸志珲, 等. 微生物降解石油污染物机制研究进展[J]. 生物技术通报, 2018, 34(10):1-9.

    HUA T, LI S N, DI Z H, et al. Review on mechanism and application of microbe degrading petroleum pollutants[J]. Biotechnology Bulletin, 2018, 34(10):1-9(in Chinese).

    [79] KASAI Y, KISHIRA H, SASAKI T, et al. Predominant growth of Alcanivorax strains in oil-contaminated and nutrient-supplemented sea water[J]. Environmental Microbiology, 2002, 4(3):141-147.
    [80] NIKOLOPOULOU M, KALOGERAKIS N. Enhanced bioremediation of crude oil utilizing lipophilic fertilizers combined with biosurfactants and molasses[J]. Marine Pollution Bulletin, 2008, 56(11):1855-1861.
    [81] BOUFADEL M C, GENG X, SHORT J. Bioremediation of the exxon valdez oil in prince william sound beaches[J]. Marine Pollution Bulletin, 2016, 113(1-2):156-164.
    [82] BELLAGAMBA M, VIGGI C C, ADEMOLLO N, et al. Electrolysis-driven bioremediation of crude oil-contaminated marine sediments[J]. New Biotechnology, 2017, 38(7):84-90.
    [83] STELTING S, BURNS R G, SUNNA A, et al. Immobilization of Pseudomonas sp. strain ADP:A stable inoculant for the bioremediation of atrazine[J]. Applied Clay Science, 2012, 64(8):90-93.
    [84] LIN J, GAN L, CHEN Z, et al. Biodegradation of tetradecane using Acinetobacter venetianus immobilized on bagasse[J]. Biochemical Engineering Journal, 2015, 100(8):76-82.
    [85] GENTILI A R, CUBITTO M A, FERRERO M, et al. Bioremediation of crude oil polluted seawater by a hydrocarbon-degrading bacterial strain immobilized on chitin and chitosan flakes[J]. International Biodeterioration & Biodegradation, 2006, 57(4):222-228.
    [86] WANG P, YUAN Y, LI Q, et al. Isolation and immobilization of new aerobic denitrifying bacteria[J]. International Biodeterioration & Biodegradation, 2013, 76(1):12-17.
    [87] 陈远. 固定化威尼斯不动杆菌与异相Fenton体系处理水体中柴油污染[D]. 福州:福建师范大学, 2017. CHEN Y. Remediation of diesel pollution in water bodies using immobilized Acinetobacter venetianus and a heterogenous Fenton system[D]. Fuzhou:Fujian Normal University, 2007(in Chinese).
    [88] LIU P W G, LIOU J W, LI Y T, et al. The optimal combination of entrapped bacteria for diesel remediation in seawater[J]. International Biodeterioration & Biodegradation, 2015, 102(6):383-391.
    [89] SAEKI H, SASAKI M, KOMATSU K, et al. Oil spill remediation by using the remediation agent JE1058BS that contains a biosurfactant produced by Gordonia sp. strain JE-1058[J]. Bioresource Technology, 2008, 100(2):572-577.
    [90] 王鑫, 王学江, 卜云洁, 等. 漂浮型固定化微生物去除海洋石油污染物研究[J]. 水处理技术, 2014, 40(9):48-51.

    WANG X, WANG X J, BU Y J, et al. Study on removing marine petroleum pollutants by floating immobilized microorganism[J]. Technology of Water Treatment, 2014, 40(9):48-51(in Chinese).

  • 加载中
计量
  • 文章访问数:  1976
  • HTML全文浏览数:  1976
  • PDF下载数:  49
  • 施引文献:  0
出版历程
  • 收稿日期:  2019-04-09
  • 刊出日期:  2019-12-10

强化微生物修复石油污染海洋的研究进展

    通讯作者: 刘雅娟, E-mail: dtdxliuyajuan@139.com ;  赵建国, E-mail: jgzhaoshi@163.com
  • 1. 山西大同大学化学与环境工程学院, 大同, 037009;
  • 2. 山西大同大学炭材料研究所, 大同, 037009
基金项目:

山西省回国留学人员资助项目(2017-108)和大同市重点研发计划(2018020)资助.

摘要: 

海洋石油污染使石油烃(Petroleum hydrocarbons,PHCs)成为海洋重要有机污染物.快速有效清除石油污染物是当前急需解决的海洋环境问题之一.利用石油烃降解菌(Petroleum hydrocarbon-degrading bacteria,PHDB)进行的原位微生物修复是一种对环境友好且较经济的清除石油污染场所和管理海洋表面溢油的有效技术.本文综述了石油烃降解菌的特性和对环境的适应性.重点分析探讨了引入外源微生物,投加生物表面活性剂、添加营养物质和增加溶解氧含量、采用微生物固定化技术等方法强化原位微生物修复石油污染海洋的机制和研究进展.展望了海洋石油污染微生物修复领域的研究前景.

English Abstract

参考文献 (90)

目录

/

返回文章
返回