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新型抗菌剂——群体感应抑制剂与传统抗菌剂对大肠杆菌的联合毒性效应

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刘艺楠, 孙昊宇, 沈洪艳, 林志芬. 新型抗菌剂——群体感应抑制剂与传统抗菌剂对大肠杆菌的联合毒性效应[J]. 环境化学, 2020, (4): 941-949. doi: 10.7524/j.issn.0254-6108.2019040104
引用本文: 刘艺楠, 孙昊宇, 沈洪艳, 林志芬. 新型抗菌剂——群体感应抑制剂与传统抗菌剂对大肠杆菌的联合毒性效应[J]. 环境化学, 2020, (4): 941-949. doi: 10.7524/j.issn.0254-6108.2019040104
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LIU Yinan, SUN Haoyu, SHEN Hongyan, LIN Zhifen. Combined toxicities of a new antimicrobials-quorum sensing inhibitors and traditional antimicrobials against Escherichia coli[J]. Environmental Chemistry, 2020, (4): 941-949. doi: 10.7524/j.issn.0254-6108.2019040104
Citation: LIU Yinan, SUN Haoyu, SHEN Hongyan, LIN Zhifen. Combined toxicities of a new antimicrobials-quorum sensing inhibitors and traditional antimicrobials against Escherichia coli[J]. Environmental Chemistry, 2020, (4): 941-949. doi: 10.7524/j.issn.0254-6108.2019040104
shu

新型抗菌剂——群体感应抑制剂与传统抗菌剂对大肠杆菌的联合毒性效应

  • 1. 河北科技大学环境与工程学院, 石家庄, 050011;

  • 2. 污染控制与资源化研究国家重点实验室, 同济大学环境科学与工程学院, 上海, 200092

    • 通讯作者: 沈洪艳, E-mail: shy0405@sina.com ; 
  • 基金项目:

    国家自然科学基金（41373096，21577105，21777123），国家环保公益课题（201509041-05），河北省自然科学基金（B2014208068），河北省药用分子化学实验室开放基金，河北省环保厅公益课题，河北省重点学科建设基金，同济大学污染控制与资源化研究国家重点实验室自主研究（重点）项目（PCRRK16007），水体污染控制与治理科技重大专项（2018ZX07109-1），上海市科学技术委员会科研计划课题（17DZ1200103，14DZ2261100），环境化学与生态毒理学国家重点实验室开放基金课题（KF2016-11）和111工程资助.

Combined toxicities of a new antimicrobials-quorum sensing inhibitors and traditional antimicrobials against Escherichia coli

  • 1. College of Environmental Science and Engineering, Hebei University of Technology, Shijiazhuang, 050011, China;

  • 2. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China

    • Corresponding author: SHEN Hongyan, shy0405@sina.com ; 
  • Fund Project: Supported by the National Natural Science Foundation of China (41373096,21577105,21777123), National Environmental Protection Project (201509041-05), Hebei Province Natural Science Fundation (B2014208068), Hebei Province Pharmaceutical Molecular Chemistry Laboratory Open Foundation, Hebei Province Environmental Protection Public Interest Project, and Hebei Province Key Disciplines Construction Fund Project, the Foundation of the State Key Laboratory of Pollution Control and Resource Reuse, China (PCRRK16007), the National Water Pollution Control and Treatment Science and Technology Major Project of China (2018ZX07109-1), the Science & Technology Commission of Shanghai Municipality (17DZ1200103,14DZ2261100), the State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2016-11) and the 111 Project.

  • 摘要: 群体感应抑制剂（QSIs）作为一种抗生素的可能代替品，具有广阔的应用前景.一些研究指出QSIs与传统抗菌药物联合使用可以在提高药效的同时降低细菌耐药性.随着QSIs使用量地逐渐增大，在环境中可能与传统抗菌剂以混合的形式存在，因此有必要研究QSIs与传统抗菌剂的潜在联合毒性效应.但是，在目前的相关联合毒性效应研究中，被研究的传统抗菌剂种类有限，传统抗菌剂与QSIs的联合毒性效应可能由于传统抗菌剂的不同而发生改变.本文以大肠杆菌为受试生物，以磺胺和银类抗菌剂作为传统抗菌剂的代表，研究了两类传统抗菌剂与QSIs的二元以及三元联合毒性效应.结果表明，在磺胺-QSIs的30组二元联合毒性效应中，有24组表现为拮抗，6组表现为相加；在银类抗菌剂-QSIs的10组二元联合毒性效应中，有6组表现为拮抗，4组表现为相加；在磺胺-银类抗菌剂-QSIs的60组三元联合毒性效应中，有47组表现为拮抗，13组表现为相加.通过对比2种银类抗菌剂与QSIs的二元联合毒性效应，可以看出2种银类抗菌剂提供制毒部分的可能均为银离子；并且由于银类抗菌剂能最快地进入细菌细胞并完成与巯基的结合进而发挥药效，所以在三元混合中，银类抗菌剂可能起主要作用.
    Abstract: Quorum sensing inhibitors (QSIs), as a new class of antimicrobials with broad prospects of application, are considered as the ideal alternatives of traditional antimicrobials. Some researchers suggested to use the combination of QSIs and traditional antimicrobials, which could not only ensure the antibacterial efficiency of traditional antimicrobials but also reduce the occurrence of antimicrobial resistance. However, in the studies on the joint effects of traditional antimicrobials and QSIs the number of the tested traditional antimicrobials is very limited. The joint effects of traditional antimicrobials and QSIs may change with the types of traditional antimicrobials. In this paper, Escherichia coli was used as the test organism, and sulfanilamide as well as silver antimicrobials were selected as the representative of traditional antimicrobials. Binary and ternary joint effects of the two types of traditional antimicrobials and QSIs were studied. The results showed that in the 30 groups of sulfanilamide-QSIs binary mixtures, 24 groups were antagonistic, and 6 groups were additive. In the 10 groups of silver antimicrobials-QSIs binary mixtures, 6 groups were antagonistic and 4 groups were additive. In the 60 groups of sulfanilamide-silver antimicrobials-QSIs ternary mixtures, 47 groups were antagonistic and 13 groups were additive. By comparing the binary joint effects of the two silver antimicrobials and QSIs, it could be hypothesized that the silver antimicrobials might exert their toxic effects though their sliver ions. In addition, silver antimicrobials seemed to play a main role in the ternary mixtures, and the probable reason is that silver antimicrobials could enter the bacterial cells rapidly and bind with the mercapto group afterwards.
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  • 收稿日期:  2019-04-01

新型抗菌剂——群体感应抑制剂与传统抗菌剂对大肠杆菌的联合毒性效应

    通讯作者: 沈洪艳, E-mail: shy0405@sina.com ; 
  • 1. 河北科技大学环境与工程学院, 石家庄, 050011;
  • 2. 污染控制与资源化研究国家重点实验室, 同济大学环境科学与工程学院, 上海, 200092
  • 收稿日期:  2019-04-01
基金项目:

国家自然科学基金（41373096，21577105，21777123），国家环保公益课题（201509041-05），河北省自然科学基金（B2014208068），河北省药用分子化学实验室开放基金，河北省环保厅公益课题，河北省重点学科建设基金，同济大学污染控制与资源化研究国家重点实验室自主研究（重点）项目（PCRRK16007），水体污染控制与治理科技重大专项（2018ZX07109-1），上海市科学技术委员会科研计划课题（17DZ1200103，14DZ2261100），环境化学与生态毒理学国家重点实验室开放基金课题（KF2016-11）和111工程资助.

摘要: 群体感应抑制剂（QSIs）作为一种抗生素的可能代替品，具有广阔的应用前景.一些研究指出QSIs与传统抗菌药物联合使用可以在提高药效的同时降低细菌耐药性.随着QSIs使用量地逐渐增大，在环境中可能与传统抗菌剂以混合的形式存在，因此有必要研究QSIs与传统抗菌剂的潜在联合毒性效应.但是，在目前的相关联合毒性效应研究中，被研究的传统抗菌剂种类有限，传统抗菌剂与QSIs的联合毒性效应可能由于传统抗菌剂的不同而发生改变.本文以大肠杆菌为受试生物，以磺胺和银类抗菌剂作为传统抗菌剂的代表，研究了两类传统抗菌剂与QSIs的二元以及三元联合毒性效应.结果表明，在磺胺-QSIs的30组二元联合毒性效应中，有24组表现为拮抗，6组表现为相加；在银类抗菌剂-QSIs的10组二元联合毒性效应中，有6组表现为拮抗，4组表现为相加；在磺胺-银类抗菌剂-QSIs的60组三元联合毒性效应中，有47组表现为拮抗，13组表现为相加.通过对比2种银类抗菌剂与QSIs的二元联合毒性效应，可以看出2种银类抗菌剂提供制毒部分的可能均为银离子；并且由于银类抗菌剂能最快地进入细菌细胞并完成与巯基的结合进而发挥药效，所以在三元混合中，银类抗菌剂可能起主要作用.

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