目的 基于传质数学模型，探索丹参-枳实复方中成分存在状态与纳滤分离机制。方法 以原儿茶醛、迷迭香酸、丹酚酸B、辛弗林为指标，进行单体成分溶液及丹参-枳实复方溶液纳滤分离，收集膜通量与指标成分截留率，基于传质模型中传质系数与溶质浓度的幂值相关性，以单一成分为参照，拟合复方溶液中成分存在状态，探讨纳滤分离机制。结果 分离压力与膜通量呈正性相关，指标成分在单体溶液和复方溶液中传质系数与浓度幂值相关性高，回归系数均大于0.98，纳滤传质模型成立。通过单一成分传质特征构建复方溶液中成分状态解析模式，丹参-枳实复方溶液中指标成分以复合态存在的比例为原儿茶醛26.17%、迷迭香酸79.87%、丹酚酸B 89.15%，辛弗林96.92%。结论 构建了复方溶液的成分存在状态解析模式，阐明了丹参-枳实复方提取液纳滤分离机制，为中药成分分离精制提供技术支撑。

Objective To explore the molecular state and nanofiltration separation mechanism of Danshen (Salviae Miltiorrhizae Radix et Rhizoma, SMRR)-Zhishi (Aurantii Fructus Immaturus, AFI) complex solution by mass transfer mathematical model. Methods In the experiment, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, synephrine were selected as indexes to analyze membrane flux and rejection in single component solution and SMRR-AFI complex solution. Based on the power correlation between mass transfer coefficient and solute concentration, the molecular state of components in the complex solution was fitted with single component. And then, the mechanism of nanofiltration separation was discussed with the molecular state in complex solution. Results The separation pressure was positively correlated with membrane flux. The mass transfer coefficients and solute concentration had high power correlation in single component solution and complex solution, and the nanofiltration mass transfer model was established successfully with the regression coefficients (R² > 0.98). The composite state proportion of index components in the complex solution was protocatechuic aldehyde of 26.17%, rosmarinic acid of 79.87%, salvianolic acid B of 89.15% and synephrine of 96.92%. Conclusion The analytical model of molecular state is established to clarify the nanofiltration separation mechanism in SMRR-AFI complex solution, which provides technical support for the separation and refining of Chinese medicinal ingredients.

R283.6

国家自然科学基金资助项目（82074006）；中药制药过程新技术国家重点实验室开放基金项目（SKL2020Z0101）；2020年国家级大学生创新创业训练计划项目（202010315037）