[关键词]
[摘要]
目的 采用活性指导分离的方法研究百合科藜芦属植物Veratrum virid和藜芦V.nigrum干燥根及根茎的抗真菌活性成分。方法 利用多种色谱技术进行分离纯化。通过现代波谱技术对单体化合物进行结构鉴定。结果 从V.virid的甲醇提取物中分离得到10个化合物,分别鉴定为rubivirine-3-O-β-glucopyranoside(1)、veratrosine(2)、veratramine(3)、pseudojervine(4)、rubivirine(5)、resveratrol 3,4'-O,O'-di-β-D-glucopyranoside(6)、mulberroside A(7)、resveratrol 4'-O-β-D-glucopyranoside(8)、resveratrol 3-O-β-D-glucopyranoside(9)、oxyresveratrol-2-O-β-D-glucopyranoside(10);从藜芦的甲醇提取物中分离得到9个化合物,分别鉴定为veratrosine(2)、veratramine(3)、pseudojervine(4)、rubivirine(5)、verussurinine(11)、veralosidine(12)、jervine(13)、γ-chaconine(14)、germidine(15)。结论 其中化合物1~5、11~15为甾体生物碱类化合物,6~10为二苯乙烯苷类化合物。化合物1为新的甾体生物碱类化合物;化合物5~10、14为首次从该属植物中分离得到。采用纸片扩散法评价化合物的抑真菌活性,结果显示化合物14对新生隐球菌和克柔念珠菌都具有较好的抑制活性,半数抑制浓度(IC50)分别为4.1、19.8μg/mL。并且化合物14在100 μmol/L浓度下对4株人肿瘤细胞都不具有抑制活性。
[Key word]
[Abstract]
Objective To study the antifungal compounds from the roots and rhizomes of Veratrum virid and V. nigrum by using the method of bioassay-guided isolation. Methods Various column chromatographic techniques were used for isolation and purification from the roots and rhizomes of V. virid and V. nigrum, and the structures of the compounds were identified on the basis of spectroscopic data. Results Ten compounds were isolated from the MeOH extract of the roots and rhizomes of V. virid, and the structures were identified as rubivirine-3-O-β-glucopyranoside (1), veratrosine (2), veratramine (3), pseudojervine (4), rubivirine (5), resveratrol 3,4'-O,O'-di-β-D-glucopyranoside (6), mulberroside A (7), resveratrol 4'-O-β-D-glucopyranoside (8), resveratrol 3-O-β-D-glucopyranoside (9), and oxyresveratrol-2-O-β-D-glucopyranoside (10); And nine compounds were isolated from the MeOH extract of the roots and rhizomes of V. nigrum, and the structures were identified as veratrosine (2), veratramine (3), pseudojervine (4), rubivirine (5), verussurinine (11), veralosidine (12), jervine (13), γ-chaconine (14), and germidine (15). Conclusion Among them, compounds 1—5, 11—15 are steroidal alkaloids, compounds 6—10 are stilbene glycosides. Compound 1 is a new compound, compounds 5—10, 14 are firstly isolated from the genus Veratrum. Moreover, the antifungal activity of compounds 1—15 are tested and evaluated by the CLSI disk diffusion method. Compound 14 displays potent antifungal activity against Canidia neoformans and C. krusei with IC50 values of 4.1 μg/mL and 19.8 μg/mL, respectively. And compound 14 shows no cytotoxicity against these four human cancer cell lines at 100 μmol/L.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金资助项目(81873071);广东省基础与应用基础研究基金项目(2021A1515011606);广州市科技计划珠江科技新星专项(201906010059);急救与创伤研究教育部重点实验室(海南医学院)开放课题基金(Grant.KLET-201911)