[关键词]
[摘要]
目的 探究槐定碱对永久性大脑中动脉栓塞(permanent middle cerebral artery occlusion,pMCAO)大鼠脑细胞的作用及机制。方法 SD大鼠随机分为对照组、模型组和槐定碱低、中、高剂量(2.5、5.0、10.0 mg/kg)组,给予槐定碱5 d后,建立pMCAO大鼠模型。考察槐定碱对pMCAO大鼠神经功能评分的影响;考察槐定碱对pMCAO大鼠脑梗死率的影响;采用流式细胞术检测各组大鼠缺血半暗带区细胞凋亡率;采用苏木素-伊红(HE)染色法观察各组大鼠脑组织病理变化;采用Western blotting法考察各组大鼠脑组织B细胞淋巴瘤-2(B-cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达情况。结果 与模型组比较,槐定碱组大鼠神经缺陷症状改善,脑梗死率显著降低(P<0.05、0.01),缺血半暗带细胞凋亡率明显降低(P<0.05、0.01),脑组织损伤有所改善,脑组织Caspase-3和Bax蛋白表达水平显著降低(P<0.05、0.01),Bcl-2蛋白表达水平显著升高(P<0.05、0.01)。结论 槐定碱对pMCAO大鼠脑细胞具有保护作用,其机制可能与上调Bcl-2及下调Bax、Caspase-3蛋白表达水平,进而抑制细胞凋亡有关。
[Key word]
[Abstract]
Objective To explore the effect and mechanism of sophoridine on brain cells in rats with permanent middle cerebral artery occlusion (pMCAO). Methods SD rats were randomly divided into control group, model group, low-, medium- and high- dose (2.5, 5.0, 10.0 mg/kg) sophoridine groups. After ip sophoridine for 5 d, pMCAO rat model was established. Effect of sophoridine on neurological function score in pMCAO rats were investigated; Effect of sophoridine on cerebral infarction rate in pMCAO rats were investigated; Flow cytometry was used to detect the cell apoptosis rate in ischemic penumbra of rats in each group; Hematoxylin-eosin (HE) staining method was used to observe the pathological changes in brain tissue of rats in each group; Western blotting was used to investigate expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), and Caspase-3 in brain tissue of rats. Results Compared with model group, neurological deficit symptoms of rats in sophoridine group were improved, cerebral infarction rate was significantly reduced (P < 0.05, 0.01), cell apoptosis rate in ischemic penumbra was significantly reduced (P < 0.05, 0.01), tissue damage was improved, expressions of Caspase-3 and Bax in brain tissue were significantly reduced (P < 0.05, 0.01), and Bcl-2 expression was significantly increased (P < 0.05, 0.01). Conclusion Sophoridine has a protective effect on brain cells in pMCAO rats, its mechanism may be related to up-regulation of Bcl-2 and down-regulation of Bax and Caspase-3 expressions, thereby inhibiting cell apoptosis.
[中图分类号]
R285.5
[基金项目]
石家庄市科学技术研究与发展指导计划项目(161460713)