目的 研究紫花鄂北贝母Fritillaria ebeiensis var. purpurea外植体愈伤组织诱导的激素组合和配比, 并考察原药材及愈伤组织中总生物碱的量。方法 以紫花鄂北贝母新鲜鳞茎为外植体, 用抗生素和NaClO组合作消毒剂, 基于MS固体培养基添加激素2,4-D+6-BA、2,4-D+KT设计正交试验进行离体培养;采用可见-紫外分光光度法测定总生物碱量。结果 外植体适宜灭菌条件是以5% NaClO溶液消毒8 min;愈伤组织诱导激素水平为2,4-D 0.5 mg/L+6-BA 2.0 mg/L;原药材及其愈伤组织中总生物碱量分别为1.46%、1.09%。结论 愈伤组织质地疏松, 适合继代培养, 其有效成分总生物碱量低于原药材。

Objective To study the combination and proportion for the explants in NaClO and the suitable hormone induced by callus of Fritillaria ebeiensis var. purpurea. The total alkaloids content variations of raw herb and callus were investigated. Methods The fresh bulbs of F. ebeiensis var. purpurea were used as the exprimental materials and NaClO and antibiotics as disinfectants. It was cultured in vitro in MS medium with plant hormones 2,4-D + 6-BA and 2,4-D + KT by orthogonal test design. Alkaloids contents in original bulb and callus were measured with UV. Results The best way to sterilization was at a concentration of 5% NaClO with 8 min. The optimal hormone levels was 2,4-D 0.5 mg/L + 6-BA 2.0 mg/L. The alkaloid contents of raw herbs and callus were 1.46% and 1.09%. Conclusion Callus induced by tissue culture technique are soft, luster, and transparent, which are suitable for subculture. Total alkaloids content in callus is less than that in raw herb.

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