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Chinese Journal of Hospital Pharmacy. 2014, (18) Core

Study on the interactions of three kinds of Pulsatilla chinensis saponins against Schistosoma japonicum with isobolographic analysisChinese Full Text

SUN Zhen;WU De-zhi;MA Zheng;XIA Chao-ming;XU Qiong-ming;ZHU Wei-feng;Key Laboratory of Modern Preparation of TCM,Ministry of Education,Jiangxi University of Traditional Chinese Medicine;Soochow University;

Abstract: OBJECTIVE To study the interactions of three kinds of Pulsatilla chinensis saponins against Schistosoma japonicum with isobolographic analysis.METHODS The adults of Schistosoma japonicum were randomly divided into following groups:B3 group（A group）,B7 group（B group）,B9 group（C group）,B3/B7group（D group）,B3/B9group（E group）,B7/B9group（F group）,B3/B7/B9group（G group）.With the rate of reduced vitality as the index,different doses of the monomer and composite drug were given for every group to calculate the ED50（Median effective dose）by the software of SPASS11.5,and the interaction between the three kinds of drugs were analyzed by the graph of isobolographic analysis.RESULTSThe ED50 of group A was 21.13μg·ml-1（The 95%of confidence limits was 14.96-30.96μg·ml-1）,The ED50 of group B was 2.56μg·ml-1（The 95%of confidence limits was 1.89-3.12μg·ml-1）,The ED50 of group C was 15.00μg·ml-1（The95%of confidence limits was 10.68-21.77μg·ml-1）,The ED50 of group D was 11.02/1.92μg·ml-1（The 95% of confidence limits was 8.05/1.31-14.41/2.47μg·ml-1）,The ED50 of group E was 12.99/8.57μg·ml-1（The 95%of confidence limits was 9.43/6.10-18.02/12.22μg·ml-1）,The ED50 of group F was 1.84/2.42μg·ml-1（The 95%of confidence limits was 1.33/1.40-2.21/3.85μg·ml-1）,The ED50 of group G was 3.56/2.39/2.52μg·ml-1（The 95% of confidence limits was 1.17/1.91/1.00-6.57/2.89/5.25μg·ml-1）.At the end of the effect of the against Schistosoma japonicum,the ED50 of group F and G had significant difference（P＜0.05）,deviating the sum of line,but the group D and E not（P＞0.05）.CONCLUSION Isobologram is a suitable and convenient way to reveal the interaction between the three kinds of drugs for against Schistosoma japonicum.In this study,we find that the Pulsatilla chinensis saponin B3/B7（6∶1）and the B3/B9（3∶2）have a additive effect,while the B7/B9（1∶1.5）and B3/B7/B9（2∶1∶1.5）have a synergistic effect,the mixture after optimizing is superior to ordinary extract against Schistosoma japonicum.
Keywords：

Pulsatilla chinensis saponins; isobolographic analysis; interaction; Schistosoma japonicum;

  • DOI：

    10.13286/j.cnki.chinhosppharmacyj.2014.18.03

  • Series：

  • Subject：

  • Classification Code：

    R285.5

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