Study on enzymatic hydrolysis conditions of glucosinolate in Capparis spinosa L.fruit and identification on the productsChinese Full Text
XIAN Yuan-yuan;ZHANG Hua;WU Zen-bao;HAN Bo;CHEN Wen;Shihezi University;
Abstract: OBJECTIVE To research the impact of factors of enzymatic hydrolysis for glucosinolate in Capparis spinosa fruit;to determine the optimum conditions of enzymatic hydrolysis and identify the enzymatic hydrolysis products.METHODS Taking the Capparis spinosafruit powder as the material,the different temperature,pH and time of single factor test and orthogonal test were studied to optimize the best conditions of the hydrolysis of glucosinolate;and the enzymatic hydrolysis products were identified by GC-MS.RESULTS The results showed that the optimum enzymatic hydrolysis conditions for the glucosinolate were as follows:enzymatic time 140 min,temperature 50℃,pH7.0,and three isothiocyanate compounds were identified from the enzymatic hydrolysis products of the glucosinolate by GC-MS and they accounted for 80% of the whole volatile products.CONCLUSION This paper identified the optimum conditions of the enzymatic hydrolysis for glucosinolate in Capparis spinosafruit and provided the theoretical basis of study on Capparis spinosafruit.
- DOI：
10.13286/j.cnki.chinhosppharmacyj.2014.15.04
- Series：
- Subject：
- Classification Code：
R29
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