Construction of ACPP-pHPMA-Ad.egfp conjugate and study on its ability to evade neutralising antibodyChinese Full Text
LI Shu-hua;CHEN Ting-ting;LIU Yu-kun;LI Hao;CHEN Xian-lian;ZHANG Ya-jie;Guangzhou Medical University;
Abstract: OBJECTIVE To synthesize and characterize an ACPP and multivalent hydrophilic HPMA polymer-coated adenovirus conjugate,for increase the resistance of adenovirus to antibody neutralization.METHODS A random poly-[N-(2-hydroxypropyl)methacrylamide](pHPMA)and activable cell-penetrating peptide(ACPP)were prepared by free radical precipitation copolymerization method,polymer modification of virus was performed by incubating ACPP、pHPMA and adenovirus in 10% glycerol/PBS(pH7.8)at 4℃for 12 h to form ACPP-pc-Ad.egfp conjugates;Particle size after virus modification was measured by dynamic light scattering(DLS);the infected ability of ACPP-pc-Ad.egfp was measured atλex488 nm andλem 538 nm in a Fluoroskan plate reader,and virus neutralisation assay of ACPP-pc-Ad.egfp was examined by measuring gfp expression in A549 cells.RESULTS pHPMA and peptides ACPP were synthesized as devised and ACPP-pc-Ad.egfp was prepared successfully,the average diameter of ACPP-pc-Ad.egfp was bigger than that of Ad.egfp and pc Ad.egfp demonstrated that efficient virus modification have been achieved;gfp expression measured in a fluorescence plate reader indicated that ACPPpc-Ad.egfp conjugates kept the ability to infect A549 cells and virus neutralisation assays showed that ACPP-pc-Ad.egfp was 9-fold more resistant to neutralisation than Ad.egfp。CONCLUSION Polymer-coated adenovirus ACPP-pc-Ad.egfp was successfully synthesized,the virus neutralisation assay Results showed ACPP-pc-Ad.egfp could evade neutralising antibody efficiently.
Keywords:
- DOI:
10.13286/j.cnki.chinhosppharmacyj.2014.11.01
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- Classification Code:
R392
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