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Chinese Journal of Hospital Pharmacy. 2014, (08) Core

Effects of PAMd on Calpain activities of cortex and hippocampus in the ischemia-reperfusion ratsChinese Full Text

CHEN Dang;ZHOU Mei;SUN Tie;FENG Xiu-ling;GUO Lian-jun;Department of Neurology,Yingshan Hospital;Department of Pharmacology,School of Basic Medical Sciences,Tongji Medical College,Huazhong University of Science and Technology;

Abstract: OBJECTIVE To investigate the effect of PAMd on Calpain activities in focal cerebral ischemia（ 2h） reperfusion（ 24h） model of rats. METHODS The animal model was induced by transient occlusion of the middle cerebral artery,PAMd was given immediately after reperfusion,then it was given in 22 h after reperfusion again. The dose of PAMd was 5,10,20 mg·kg-1,respectively. The activities of Calpain in hippocampus and cortex were determined by spectrophotography. RESULTS The Calpain activities of ischemic cortex increased significantly to（ 2. 81 ± 0. 38） △A·mg-1（ protein）,and the Calpain activity of normal cortex was（ 1. 68 ±0. 21） △A·mg-1（ protein）. Given PAMd（ 10,20 mg·kg-1） after I-R,the Calpain activities of ischemic cortex decreased to（ 2. 06 ±0. 47） and（ 1. 98 ±0. 34） △A·mg-1（ protein） respectively. The Calpain activities of ischemic hippocampus increased significantly to（ 2. 96 ± 0. 41） △A·mg-1（ protein）. Given PAMd（ 10,20 mg·kg-1） after I-R,the Calpain activities of ischemic hippocampus decreased to（ 2. 28 ± 0. 29） and（ 2. 08 ± 0. 34） △A·mg-1（ protein） respectively. CONCLUSION The results showed above proved that PAMd could decrease the Calpain activities of ischemic cortex and hippocampus in I-R rats,then reduce the apoptosis or death of neurons after brain ischemia.
Keywords：

phenolic alkaloids from Menispermum dauricum; brain ischemia; Calpain; cortex; hippocampus;

  • DOI：

    10.13286/j.cnki.chinhosppharmacyj.2014.08.06

  • Series：

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  • Classification Code：

    R285.5

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