Knowledge Network Node
Progress in Modern Biomedicine. 2014, (26)

Curcumin Through Reduced Ets-1 Expression Induced Inhibition of AngⅡHeart into Fibroblast ProliferationChinese Full Text

YANG Hui-yu;PAN Jin;LI Ping;TANG Rui;XIANG Meng;Department of internal medicine,Clinical Medical College of Xi’an Medical University;

Abstract: Objective: To observe the inhibition effect of curcumin（Cur） on AngⅡ-induced over-proliferation and overexpression of CTGF and PAI-1 in CFs and relation between its inhibitory effect and the suppressing expression of Ets-1. Methods: Ets-1 were measured by real-time PCR and western-blotting, respectively. After transfected with Ets-1 si-RNA, the cells were incubated with AngII（100 nmol/L） for indicated times. The cell activity was detected by MTT assay and the levels of mRNA and protein of CTGF, PAI-1,Ets-1 were measured by real-time PCR and western-blotting, respectively. Results: With the lower expression of Ets-1, the proliferation which were induced by AngⅡ was decreased in CFs cells and the expression of CTGF and PAI-1 also declined. Curcumin can significantly reduced the expression of Ets-1 and the reduction was also concentration-related（P＜0.05）. Conclusion: Curcumin significantly inhibits AngⅡ-induced overexpression of Ets-1 in the levels of mRNA and protein in CFs, and Ets-1 siRNA also has the same inhibitory effect as Cur. These results suggested that Cur’s anti-fibrotic effect, in part, depends on its inhibitory effect on the overexpression of Ets-1 in AngⅡ-stimulated CFs.
Keywords：

Ets-1; Curcumin; Angiotensin Ⅱ; Cardiac fibroblast;

  • DOI：

    10.13241/j.cnki.pmb.2014.26.012

  • Series：

  • Subject：

  • Classification Code：

    R285

  • Mobile Reading
    Read on your phone instantly
    Step 1

    Scan QR Codes

    "Mobile CNKI-CNKI Express" App

    Step 2

    Open“CNKI Express”

    and click the scan icon in the upper left corner of the homepage.

    Step 3

    Scan QR Codes

    Read this article on your phone.

  • HTML
  • CAJ Download
  • PDF Download

Download the mobile appuse the app to scan this coderead the article.

Tips: Please download CAJViewer to view CAJ format full text.

Download: 81 Page: 5045-5048 Pagecount: 4 Size: 689K

Related Literature
  • Similar Article
  • Reader Recommendation
  • Associated Author