Knowledge Network Node
Progress in Modern Biomedicine. 2014, (26)

Effects of Ginkgo Biloba Extract on Hydrogen Peroxide-stimulated Vascular Endothelial CellsChinese Full Text

WANG Ru-ke;SUN Yuan-yuan;HAN Jian-yi;XIAO Yao;SHI Huai-zhang;First affiliated hospital of Harbin Medical University;

Abstract: Objective: To investigate the protective effects of the Ginkgo biloba extract on vascular endothelial cells and its possible mechanisms. Methods: The endothelial cells of blood vessels were cultured. The endothelial cells apoptosis model was established by treatment with hydrogen peroxide（H2O2）. Then divide the cells into four groups: blank control group, H2O2-treated group,single ginkgo biloba extract-treated group, ginkgo biloba extract pretreatment group（giving H2O2 at 2 h after administration of ginkgo biloba extract）. The relative vitality were determined by MTT test cells. The expressions of gene CHOP were detected by RT- PCR, and the CHOP target protein expressions were detected by western blot analysis. Results: Compared with the control, the cells apoptosis rate,CHOPmRNA relative expression and CHOP protein expression was significantly increased in the H2O2-treated group. Compared with H2O2-treated group, the cells apoptosis rate, CHOPmRNA relative expression and CHOP protein expression was significantly reduced in the ginkgo biloba extract pretreatment group（P＜0.05）. Conclusion: As an antioxidant, free-radical scavenging and anti-aging medicine,Ginkgo biloba extract may protect endothelial cells by regulating CHOP protein and selectively inhibiting excessive endoplasmic reticulum stress.
Keywords：

Vascular endothelial cells; Radical scavenger; Endoplasmic reticulum stress; Apoptosis; Ginkgo biloba extract;

  • DOI：

    10.13241/j.cnki.pmb.2014.26.010

  • Series：

  • Subject：

  • Classification Code：

    R285

  • Mobile Reading
    Read on your phone instantly
    Step 1

    Scan QR Codes

    "Mobile CNKI-CNKI Express" App

    Step 2

    Open“CNKI Express”

    and click the scan icon in the upper left corner of the homepage.

    Step 3

    Scan QR Codes

    Read this article on your phone.

  • HTML
  • CAJ Download
  • PDF Download

Download the mobile appuse the app to scan this coderead the article.

Tips: Please download CAJViewer to view CAJ format full text.

Download: 197 Page: 5038-5041+5048 Pagecount: 5 Size: 754K

Related Literature
  • Similar Article
  • Reader Recommendation
  • Associated Author