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Progress in Modern Biomedicine. 2014, (23)

Resveratrol Inhibits the Proliferation and Promotes the Apoptosis of SiHa Cells by Reducing Galectin-3 ExpressionChinese Full Text

WANG Heng;LI Ya-ling;DAI Wei-dong;WANG Ping;LI Jiang-peng;Department of Obstetrics and Gynecology, The Affiliated Guangren Hospital of Xi’an Jiaotong University College of Medicine;

Abstract: Objective: To observe the effect of resveratrol on the proliferation and apoptosis of SiHa cells, and explore the related role of galectin. Methods: Human cervical cancer cell line SiHa was cultured in vitro, the experiment was divided into 5 groups as follows: control group, resveratrol and [resveratrol+galectin-3（5 μg/mL, 10 μg/mL and 20 μg/mL）]. They were given normal saline,resveratrol and [resveratrol+galectin（5 μg/mL, 10 μg/mL and 20 μg/mL）], respectively. 48 hours later, the cells were collected. Cell proliferation was detected by MTT, cell apoptosis was determined by Caspase-3 activity assay kit, the galectin-3 expression was evaluated by Western Blot. Results: Resveratrol（300 μmol/L） obviously inhibited the proliferation of SiHa cells, significantly increased the apoptosis level, and markedly reduced galectin-3 expression. SiHa cells were given different concentrations of galectin-3（5, 10, 20μg/mL） along with resveratrol（300 μmol/L）. With the increase of galectin-3 concentration, the proliferation of SiHa cells gradually increased and the apoptosis level of SiHa cells gradually reduced, however, the phosphorylation level of VEGF-R3 gradually increased.Conclusion: Resveratrol could inhibit the proliferation and promote the apoptosis of SiHa cells via reducing the galectin-3 expression.
Keywords：

Resveratrol; Cervical cancer; galectin-3; Proliferation; Apoptosis;

  • DOI：

    10.13241/j.cnki.pmb.2014.23.013

  • Series：

  • Subject：

  • Classification Code：

    R285

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