The Correlation Study of the Plasma Diamine Oxidase Activities and the Intestinal Barrier Function in Rats with Obstructive Jaundice after Internal and External Biliary DrainagesChinese Full Text
SU Xuan;LUO Kai-fa;WU Liang;WU Li-li;LI Wen;Department of Gastroenterology the Chinese ,PLA General Hospital;
Abstract: Objective: To investigate the relationship between the plasma diamine oxidase（DAO） activities and the intestinal barrier function in rats with obstructive jaundice after internal and external biliary drainages. Methods: Sixty adult healthy male SD rats were randomly divided into four groups :obstructive jaundice group（OJ） external drainage group（ED）, internal drainage group（ID） and shame operation group（SH）. There were 15 rats in each group. Specimens from the rats of SH and OJ groups were collected one week after the surgery. Different billiary drainages were performed in ID and ED groups, and the specimens were harvested seven days after the operation. The activities of the plasma DAO were determined and the morphological changes of the terminal ileum mucosa were observed under the light microscope. The results were compared between different groups. Results: The injures of the intestinal mucosa were obvious in OJ group with thin mucosa, sparse villous, destruction of the epithelial integrity and inflammation cell infiltration, while the situations of the intestinal mucosa in ED and ID groups recovered, and ID group was more similar to SH group in intestinal mucosal morphology. Compared with the other groups, the levels of the DAO in OJ group increased dramatically（8.183±0.211 vs 3.570±0.280, 3.978±0.269, 5.051±0.328 U/L, P＜0.01）, and the activities of the DAO in ID group, which were similar to the level of SH group（P＞0. 05）, decreased more significantly than that in ED group（P＜0.01）. The changes of the plasma DAO activities were significantly correlated with pathological examination of intestinal mucosa. Conclusions: These data suggest that both of the internal and external biliary drainages may reduce the plasma DAO activities in rats with obstructive jaundice but the internal biliary drainage is better, and the level of the plasma DAO can reflect the damage of the intestinal barrier function in obstructive jaundice rats.
- DOI：
10.13241/j.cnki.pmb.2014.17.004
- Series：
- Subject：
- Classification Code：
R657.4
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