Expression Profile of Receptor Interacting Protein 3 in Different Mouse Tissues and Rat’s Myocardial Cells with Hypoxia InjuryChinese Full Text
SHAN Dong-kai;ZOU Lu-lu;SONG Xiao-wei;ZHAO Xian-xian;JING Qing;Department of Cardiology, Changhai Hospital, Second Military Medical University;
Abstract: Objective: To detect the expression profile of receptor interacting proteins（RIPs） in mouse tissues, and to detect the expression of RIP3 in rat’s myocardial cells with hypoxia injury. Methods: ① We detected the expression profile of RIPs family in mouse tissues（heart, liver, lung, kidney, brain, intestine, skeletal muscles, spleen and aorta） by real-time fluorescent quantitative PCR, then detected the expression profile of RIP3 protein. ② The myocardial cells of rat were divided into hypoxia group and control. We treated the first group for 48 h on hypoxia to obtain hypoxia injured models, then quantitated the expression of RIP3 by western blot. Results:① On mRNA level, the expression of RIP1 was highest in brain, but lower in heart, lung, kidney and skeletal muscle; the expression of RIP2 was higher in heart and lung than other tissues; RIP3 in the intestine was 4 times higher than other, but it was very low expression in brain; the expression of RIP4 was highest in lung, and was lowest in skeletal muscle, brain, spleen, and vessel. ② Western blot results showed the expression of RIP3 at protein level. It was higher expressed in brain, skeletal muscle, however, lower in heart, liver and lung. ③After treated on hypoxia, RIP3 of hypoxia group was significantly higher than control in the cultured rat myocardial cell（P＜0.05）. Conclusions: The expression of RIPs represented various level in mouse tissues. RIP3 increased in rat myocardial cells under hypoxia injured condition.
- DOI：
10.13241/j.cnki.pmb.2014.16.001
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- Classification Code：
R54
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