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Progress in Modern Biomedicine. 2014, (11)

PHD1 Inhibits NF-κB Mediated CyclinD1 Expression and Proliferation of Lung Cancer CellsChinese Full Text

XIE Xiao;MEI Ju;XIAO Hai-bo;DING Fang-bao;ZHONG Hong;ZHU Jia-quan;MA Nan;Cardiothoracic Surgical,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine;

Abstract: Objective: To find new target for lung cancer treatment through investigating the function and the mechanism of PHD1 in lung cancer cells. Methods: PHD1 was over expressed using the lipidosome or silenced using siRNA in A549 cells, then NF-κB activity was detected by luciferase assay and the expression level of cyclinD1 was measured by real-time PCR and western blot. The A549 stable cell line which expresses GFP-PHD1 was established and the cell cycle and cell growth were analyzed by flow cytometry. Finally, the growth of tumor was observed after injecting cells subcutaneously to the nude mice. Results: Overexpression of PHD1 significantly inhibited the NF-κB activity and degradation of IκBα, and decreased the mRNA and protein expression of cyclinD1; Conversely, knocking down PHD1 significantly increased the NF-κB activity and up regulated the mRNA and protein expression of cyclinD1, but it had no effect on the expression of cyclinE1. Over expression of IκBαSR inhibited the up regulation of cyclinD1 mRNA level induced by knocking down of PHD1. Over expression of PHD1 caused cell cycle arrest and significantly inhibited the cell proliferation and tumor growth. Conclusion: Over expression of PHD1 might inhibit the growth and proliferation of lung cancer cells through down-regulating the cyclinD1 expression, which was mediated by NF-κB.
Keywords：

Lung cancer; Cell proliferation; PHD1; Cyclin; NF-κB;

  • DOI：

    10.13241/j.cnki.pmb.2014.11.001

  • Series：

  • Subject：

  • Classification Code：

    R734.2

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