Construction and Significance of G0S2 Promoter-Directed Luciferase Reporter Gene PlasmidChinese Full Text
ZHANG Cui-zhen;JIANG Xue-jun;QIAN Hang;ZHANG Peng;YANG Han-dong;Department of Cardiology, Renmin Hospital of Wuhan University;Cardiovascular Research Center, Hubei University of Medicine;
Abstract: Objective: To clone the luciferase reporter gene and construct promoter region from human G0S2 gene. Methods: A region containing human G0S2 gene promoter was obtained by PCR amplification from 293A cells, and the segment was cloned into the pGL3-Basic vector and transformed into E.Coli, which was verified by endonuclease, PCR amplification and direct sequencing. The final construct（pGL3-G0S2-Promoter） was transferred into VSMC（vascular smooth muscle cells）, and the luciferase activity was measured. Results: The insert and surrounding regions in pGL3-G0S2-Promoter plasmid were confirmed. There was significant promoter activity for pGL3-G0S2-Promoter vector（P＜0.05）. Conclusions: Luciferase reporter plasmid pGL3-G0S2-Promoter containing G0S2 promoter can be built successfully. It will be used to study the transcriptional regulation of G0S2 gene.
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- DOI：
10.13241/j.cnki.pmb.2014.10.007
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- Classification Code：
Q782
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