In vitro Study on Acrylate-PEG400 Composite Membranes for Controlled Release of InsulinChinese Full Text
LI Shuo;ZHAN Xiao-ping;TANG Guo-chun;MAO Zhen-min;College of Pharmacy, Shanghai JiaoTong University;
Abstract: Objective: Among non-injection drug administration systems, transdermal drug delivery system（TDDS） has become the focus in the field of pharmaceutical research and development for its unique advantages. The study on controlled release membrane is an important component in TDDS research. However, there are few reports related to controlling insulin to release through controlled release membranes. In our laboratory, a novel acrylate-PEG400 composite membrane, which is a controlled release membrane for insulin, was synthesized by UV curing method. The aim of this experiment was to study the in vitro controlled release properties of this membrane for insulin in 24 hours, and to make a contribution to the basic research of insulin TDDS. Methods: Acrylate-PEG400 membranes were synthesized by UV curing method. Then, the controlled release properties of acrylate-PEG membranes for insulin in different concen trations were analyzed by HPLC method. The controlled release rule was studied by comparing the release properties of insulin solution in different concentrations, including cumulative permeation value and permeation rate. Results: The experimental data showed that, for three insulin solutions in different concentrations including 3.0 mg/mL, 6.0 mg/mL, 9.0 mg/mL, the related factors of controlled release curve were as follows: 0.9921, 0.9950, 0.9964. The data above indicated that this membrane could control insulin liner release well, for all the related factors were above 0.99. After calculating, the cumulative permeation values and permeation rates for three different insulin solutions, the concentrations of which were 3.0 mg/mL, 6.0 mg/mL, 9.0 mg/mL, were obtained. The cumulative permeation val ues were as follows: 266.69 μg/cm2, 343.65 μg/cm2, 460.10 μg/cm2. And the permeation rates were as follows: 9.24 μg·cm-2·h-1, 13.40 μg·cm-2·h-1, 19.04 μg·cm-2·h-1. From these two sets of data, a conclusion could be inferred that, the release rate of insulin increased by the increase of the concentration of insulin. Conclusion: From the experimental results we could see that, the acrylate-PEG400 membranes con trolled insulin to release in constant rate in vitro. After comparing the release properties of insulin including cumulative permeation value and permeation rate, a rule was found that the release rate of insulin through the acrylate-PEG400 membrane was adjustable. The specific performance of this rule was that, the release rate of insulin increased by the he increase of the concentration of insulin. Thus, this membrane could not only control insulin zero release, but also control insulin to release in a certain rate by adjusting the concentration of insulin to an appropriate proportion. So the acrylate-PEG400 membrane was an ideal controlled release membrane for insulin. This study was a basic research of controlled release membrane for insulin, which would establish a foundation for the research and development of insulin TDDS in future.
Keywords：
- DOI：
10.13241/j.cnki.pmb.2014.03.005
- Series：
- Subject：
- Classification Code：
R944.2
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