Objective To investigate the effects of sleep deprivation on intestinal oxidative stress and inflammatory response in mice. Methods Thirty-two SPF male C57 mice, aged 8-12 weeks, weighing 18-22 g, were divided into four groups using random number table method: blank control group (group C), incision group (group I), sleep deprivation group(group A), and sleep deprivation + incision group (group AI), eight mice in each group. Group C were kept normally, group I were established into incision model, group A were established into sleep deprivation model by depriving for 48 hours in a sleep deprivation chamber, and group AI were established into incision and sleep deprived models. The expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) and superoxide dismutase 2 (SOD2) protein were detected by Western blot. The fluorescence intensity of reactive oxygen species (ROS) was detected bydihydroethidium and NOX2 fluorescence intensity was detected by immunofluorescence. Results Compared with group C, the expression of IL-1β, TNF-α and NOX2 protein were significantly increased (P < 0.05), the expression of SOD2 protein was significantly decreased (P < 0.05), and ROS and NOX2 fluorescence intensity were significantly enhanced (P < 0.05) in colonic tissues in groups I, A and AI. Compared with group I, the expression of IL-1β, TNF-α and NOX2 protein of colonic tissues in groups A and AI were significantly increased (P < 0.05), the expression of SOD2 protein in group AI was significantly decreased (P < 0.05), and the fluorescence intensity of ROS and NOX2 in group AI were significantly enhanced (P < 0.05). Compared with group A, the expression of IL-1β, TNF-α and NOX2 protein were significantly increased (P < 0.05), the expression of SOD2 protein was significantly decreased (P < 0.05), and ROS and NOX2 fluorescence intensity were significantly enhanced (P < 0.05) in colonic tissues in group AI. Conclusion Sleep deprivation can cause intestinal inflammatory response and oxidative stress in mice, and the inflammatory response and oxidative stress will be further aggravated by sleep deprivation and surgery. |