In order to study the effects of dietary lipid sources on growth performance, antioxidant capacity and fatty acid composition of abdominal muscles of juvenile red swamp crayfish Procambarus clarkii. Six isonitrogenous and isoenergetic experimental diets were formulated with palm oil, fish oil and soybean oil as lipid sources. 252 juvenile P.clarkii with initial weight of(5.26±0.10) g were selected and cultured for 120 days. They were randomly assigned to 6 groups(3 replicates in each group, 14 individuals in each replicate).The results showed that the specific growth rate(SGR) and survival rate(SR) of juvenile shrimps decreased with the increase of palm oil addition level in the feed. SGR of juvenile P.clarkii in F3 and F4 groups was significantly higher than that of other groups. Survival rate of juvenile P.clarkii in F4, F5 and F6 was significantly higher than that in F1 and F3. There was no significant difference between F4, F5 and F6. The total antioxidant capacity (T-AOC) in hepatopancreas of single lipid source groups (F1, F5 and F6) was significantly higher than that of mixed lipid source groups(F2, F3 and F4). Among all feed treatments, the activity of CAT in hepatopancreas in F4 was the lowest, and the content of malondialdehyde (MDA) in F5 was the highest. There was no significant difference of ∑SFA in juvenile P.clarkii muscles of each feed group. 18 kinds of fatty acids were detected in the muscles of each group, ∑PUFA > ∑SFA > ∑MUFA, n-3/n-6 ∑PUFA values ranged from 1.44 to 1.84.Results indicated only palm oil, fish oil and soybean oil as feed lipid sources have negative effects on the growth performance and antioxidant capacity of P. clarkii,and the mixture of fish oil and soybean oil as lipid source was beneficial to SR and SGR of P.clarkii.