Phosphorous (P) is easily immobilized in acidic soil，resulting in extremely low P bioavailability.Phosphate-solubilizing bacteria (PSB) play an important role in increasing soluble phosphorus content in soils.Although there have been many studies on PSB dissolving calcium phosphate (Ca-P) in neutral to calcareous soils，few PSB strains with high efficiency of solubilizing aluminum phosphate (Al-P) in acidic soils were reported.This study first isolated PSB from the rhizosphere of different plants (Lespedeza，soybean，rice) grown in acidic soil，then compared their P ability to solubilize Ca3(PO4)2 and AlPO4，and finally explored the effects of PSB on soybean growth and P uptake.Five PSB strains were isolated and identified by 16S rRNA gene sequencing as Nguyenibacter (L1)，Pseudomonas (S1)，Serratia (S2)，Burkholderia (R1) and Ralstonia (R2).Strains S1，S2，R1 and R2 had stronger solubility for Ca-P，but weaker solubility for Al-P.Interestingly，strain L1 had stronger solubility for Al-P but weaker solubility for Ca-P.The growth and P uptake of soybean were promoted by the combined inoculation of L1 and S1 but not by the single inoculation of either L1 or S1.Taken together，the present study obtained five PSB strains with different phosphate dissolving capacity for Ca-P and Al-P，and the combined inoculation of two trains L1 and S1 significantly promoted the growth and P uptake of soybean in acidic soil.These strains can be used as microbial resources for developing high efficiency biological P fertilizer in acidic soil.