3个披碱草属牧草对低温胁迫的生理响应及苗期抗寒性评价
摘要:
探讨低温胁迫下西藏野生披碱草(Elymus)苗期抗寒性及其生理变化,可为优良牧草选育提供理论依据。以披碱草属3个牧草为试验材料,出苗21 d后在人工气候箱内进行低温胁迫,设18、10、5和0 ℃共4个低温处理,其中以18 ℃为对照。结果表明,随着低温胁迫的加剧,相对膜透性,同德老芒麦(E. sibiricus cv. Tong De)的增幅最大,巴青垂穗披碱草(E. nutans)增幅最小。叶绿素含量,巴青垂穗披碱草先升高后降低,同德老芒麦与那曲垂穗披碱草显著升高(P<0.05)。巴青垂穗披碱草可溶性蛋白(SP)含量增加幅度最小,为对照的2.59倍;同德老芒麦与那曲垂穗披碱草SP含量显著上升(P<0.05),均为对照的5.31倍。巴青垂穗披碱草与同德老芒麦的可溶性糖(SS)含量先升高后降低,而那曲垂穗披碱草SS含量显著增大(P<0.05),为对照的2.14倍。同德老芒麦的丙二醛(MDA)含量增幅最大,巴青垂穗披碱草MDA含量0 ℃较5 ℃时显著降低(P<0.05)。3种供试材料游离脯氨酸(Pro)含量均升高,同德老芒麦增幅最大,为对照的2.58倍;巴青垂穗披碱草最小,为对照的1.44倍。巴青垂穗披碱草超氧化物歧化酶(SOD)活性先增强后减弱,那曲垂穗披碱草SOD活性显著增强(P<0.05),为对照的1.65倍。那曲垂穗披碱草过氧化物酶(POD)活性上升幅度最大,为对照的4.13倍;同德老芒麦最小,为对照的2.91倍。隶属函数法综合评价表明,3种野生披碱草的抗寒性依次为巴青县野生垂穗披碱草>同德老芒麦>那曲县野生垂穗披碱草。
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